Heparin is a highly sulfated polysaccharide used extensively as an anticoagulant. However, conventional heparin extraction processes remains time-consuming and inefficient. We report cationic brush magnetic adsorbents by grafting poly(3-acrylamidopropyl) trimethylammonium chloride-‑isopropylacrylamide random copolymers onto the surface of FeO for ultrafast and efficient heparin extraction. The fabricated adsorbent demonstrated an excellent adsorption capacity of 169.36 mg g and a record-breaking adsorption rate towards heparin due to the introduction of ‑isopropylacrylamide, providing more adsorption sites and larger transport channels for the macromolecule heparin to access active sites. In addition, the adsorption capacity and rate for crude heparin were significantly better than those of commercial resin. Thermodynamic parameters implied that the heparin adsorption was spontaneous and endothermic process. The adsorption mechanism can be described by the synergistic effect of electrostatic attraction and hydrogen bond. This study paved an innovative approach to simultaneously enhance the adsorption rate and capacity of the adsorbent for polysaccharide.

中文翻译：

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通过阳离子刷磁性吸附剂超快速高效提取肝素


肝素是一种高度硫酸化的多糖，广泛用作抗凝剂。然而，传统的肝素提取工艺仍然耗时且效率低下。我们报道了通过将聚（3-丙烯酰氨基丙基）三甲基氯化铵-异丙基丙烯酰胺无规共聚物接枝到 Fe3O 表面上的阳离子刷磁性吸附剂，用于超快速、高效提取肝素。由于异丙基丙烯酰胺的引入，所制备的吸附剂表现出169.36 mg g-1的优异吸附容量和破纪录的肝素吸附率，为大分子肝素进入活性位点提供了更多的吸附位点和更大的运输通道。此外，对粗肝素的吸附能力和吸附速率明显优于商业树脂。热力学参数表明肝素的吸附是自发的吸热过程。吸附机理可以通过静电引力和氢键的协同作用来描述。这项研究为同时提高吸附剂对多糖的吸附速率和容量开辟了一条创新方法。