Rapid detection of pathogens present on contaminated surfaces is crucial for food safety and public health due to the high morbidity and mortality of bacterial infections. Herein, a sensitive and efficient method for on-site identification of foodborne pathogens on anisotropic surfaces was developed by using an in situ instantaneously prepared surface-enhanced Raman scattering (SERS) platform. To achieve this, molybdenum-doped gallic acid-derived carbon dots (MCDs) are utilized as the reductant for synthesizing Au@MCDs nanohybrids within just 3 s at ambient temperature. The synergistic effect of the electromagnetic enhancement and charge transfer of Au@MCDs enables excellent SERS performance 10 times stronger than bare Au NPs. The bioassay platform requires less than 5 min to complete the quantitative detection of foodborne pathogens on various microbial-contaminated interfaces with a sensitivity of 10 CFU/mL. This innovative strategy breaks the long-standing limitations of SERS substrates in practical use, such as the time-consuming process, interference of residual surfactants, poor surface stability, and few application scenarios, providing a promising tool for widespread applications in biomedical research and clinical diagnostics.

中文翻译：

---

瞬时制备金-碳点纳米复合材料，用于多种界面中病原体的现场 SERS 识别


由于细菌感染的高发病率和死亡率，快速检测受污染表面上存在的病原体对于食品安全和公共卫生至关重要。在此，通过使用就地瞬时制备的表面增强拉曼散射（SERS）平台。为了实现这一目标，利用钼掺杂的没食子酸衍生碳点（MCD）作为还原剂，在环境温度下仅 3 秒内合成 Au@MCD 纳米杂化物。 Au@MCD 的电磁增强和电荷转移的协同效应使其具有比裸 Au 纳米粒子强 10 倍的优异 SERS 性能。该生物测定平台仅需不到5分钟即可完成各种微生物污染界面上食源性病原体的定量检测，灵敏度为10 CFU/mL。这一创新策略打破了SERS基底在实际应用中长期以来存在的工艺耗时、表面活性剂残留干扰、表面稳定性差、应用场景少等局限性，为生物医学研究和临床的广泛应用提供了一种有前景的工具。诊断。