Third harmonic generation (THG) provides a valuable, label-free approach to imaging biological systems. To date, THG microscopy has been performed using point-scanning methods that rely on intensity measurements lacking phase information of the complex field. We report the first demonstration, to the best of our knowledge, of THG holographic microscopy and the reconstruction of the complex THG signal field with spatial synthetic aperture imaging. Phase distortions arising from measurement-to-measurement fluctuations and imaging components cause optical aberrations in the reconstructed THG field. We have developed an aberration-correction algorithm that estimates and corrects these phase distortions to reconstruct the spatial synthetic aperture THG field without optical aberrations.

中文翻译：

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合成空间孔径全息三次谐波发生显微镜


三次谐波发生 (THG) 为生物系统成像提供了一种有价值的、无标记的方法。迄今为止，THG 显微镜是使用点扫描方法进行的，这些方法依赖于缺乏复杂场相位信息的强度测量。据我们所知，我们报告了首次演示 THG 全息显微镜以及通过空间合成孔径成像重建复杂 THG 信号场。测量间波动和成像组件引起的相位失真会导致重建 THG 场中的光学像差。我们开发了一种像差校正算法，可以估计和校正这些相位失真，以重建没有光学像差的空间合成孔径 THG 场。