Various neuromodulation techniques have been developed to modulate the peak activity of neurons, thereby regulating brain function and alleviating neurological disorders. Additionally, neuronal stimulation and imaging have significantly contributed to the understanding and treatment of these diseases. Here, we propose utilizing photonic nanojets for optical stimulation and imaging of neural cells. The application of resin microspheres as microlenses enhances fluorescence imaging of neural lysosomes, mitochondria, and actin filaments by generating photonic nanojets. Moreover, optical tweezers can precisely manipulate the microlenses to locate specific targets within the cell for real-time stimulation and imaging. The focusing capabilities of these microlenses enable subcellular-level spatial precision in stimulation, allowing highly accurate targeting of neural cells while minimizing off-target effects. Furthermore, fluorescent signals during neural cell stimulation can be detected in real-time using these microlenses. The proposed method facilitates investigation into intercellular signal transmission among neural cells, providing new insights into the underlying mechanisms of neuronal cell activities at a subcellular level.

中文翻译：

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通过光子纳米喷射对神经细胞进行刺激和成像


人们已经开发了各种神经调节技术来调节神经元的峰值活动，从而调节大脑功能并减轻神经系统疾病。此外，神经元刺激和成像对这些疾病的理解和治疗做出了重大贡献。在这里，我们建议利用光子纳米喷射对神经细胞进行光刺激和成像。树脂微球作为微透镜的应用通过产生光子纳米喷射来增强神经溶酶体、线粒体和肌动蛋白丝的荧光成像。此外，光镊可以精确操纵微透镜来定位细胞内的特定目标，以进行实时刺激和成像。这些微透镜的聚焦功能可实现亚细胞级的刺激空间精度，从而能够高度准确地瞄准神经细胞，同时最大限度地减少脱靶效应。此外，可以使用这些微透镜实时检测神经细胞刺激期间的荧光信号。所提出的方法有助于研究神经细胞之间的细胞间信号传递，为亚细胞水平上神经元细胞活动的潜在机制提供新的见解。