Acrylamide (AAM), a compound extensively utilized in various industrial applications, has been reported to induce toxic effects across multiple tissues in living organisms. Despite its widespread use, the impact of AAM on ovarian function and the mechanisms underlying these effects remain poorly understood. Here, we established an AAM‐exposed mouse toxicological model using 21 days of intragastric AAM administration. AAM exposure decreased ovarian coefficient and impaired follicle development. Further investigations revealed AAM would trigger apoptosis and disturb tricarboxylic acid cycle in ovarian tissue, thus affecting mitochondrial electron transport function. Moreover, AAM exposure decreased oocyte and embryo development potential, mechanically associated with pericentrin and phosphorylated Aurora A cluster failure, leading to meiotic spindle assembly defects. Collectively, these results suggest that AAM exposure may lead to apoptosis, glucose metabolic disorders, and mitochondrial dysfunction in ovary tissue, ultimately compromising oocyte quality.

中文翻译：

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丙烯酰胺暴露会损害卵巢三羧酸循环并降低小鼠卵母细胞质量


丙烯酰胺 （AAM） 是一种广泛用于各种工业应用的化合物，据报道可在生物体的多个组织中诱导毒性作用。尽管 AAM 被广泛使用，但 AAM 对卵巢功能的影响以及这些影响背后的机制仍然知之甚少。在这里，我们使用 21 天的胃内 AAM 给药建立了一个 AAM 暴露的小鼠毒理学模型。AAM 暴露降低了卵巢系数并损害了卵泡发育。进一步研究发现，AAM 会触发细胞凋亡并扰乱卵巢组织中的三羧酸循环，从而影响线粒体电子传递功能。此外，AAM 暴露降低了卵母细胞和胚胎发育潜力，与中心粒周围和磷酸化 Aurora A 簇失败机械相关，导致减数分裂纺锤体组装缺陷。总的来说，这些结果表明，AAM 暴露可能导致卵巢组织细胞凋亡、葡萄糖代谢紊乱和线粒体功能障碍，最终影响卵母细胞质量。