Lily (Lilium spp.), a horticultural crop serving both ornamental and edible functions, derives its coloration primarily from anthocyanins. However, limited studies have been conducted on the accumulation of anthocyanins within lilies. In this study, we cloned a light-induced transcription factor named as LvBBX24 in lilies. Through genetic and biochemical analysis, we determined that LvBBX24 could upregulate the transcription of LvMYB5 and facilitate anthocyanin synthesis. Moreover, we identified that darkness promoted the degradation of LvBBX24 protein. Through screening a yeast library, we identified LvbZIP44 acts as its interacting partner. Genetic testing confirmed that LvbZIP44 also plays a role in promoting lily anthocyanin synthesis. This indicates a potential synergistic regulatory effect between LvBBX24 and LvbZIP44. Our study indicates that LvBBX24 and LvbZIP44 cooperate to regulate anthocyanin accumulation in lily petals. These findings provide compelling evidence supporting the idea that LvBBX24 and LvbZIP44 may form a looped helix surrounding the LvMYB5 promoter region to regulate anthocyanin biosynthesis.

中文翻译：

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转录因子 LvBBX24 和 LvbZIP44 在光的作用下协调花青素在百合花瓣中的积累


百合 （Lilium spp.） 是一种兼具观赏和食用功能的园艺作物，其颜色主要来自花青素。然而，关于花青素在百合体内积累的研究有限。在这项研究中，我们在百合中克隆了一种名为 LvBBX24 的光诱导转录因子。通过遗传和生化分析，我们确定 LvBBX24 可以上调 LvMYB5 的转录并促进花青素合成。此外，我们发现黑暗促进了 LvBBX24 蛋白的降解。通过筛选酵母文库，我们确定 LvbZIP44 是其相互作用的伙伴。基因检测证实 LvbZIP44 还在促进百合花青素合成中发挥作用。这表明 LvBBX24 和 LvbZIP44 之间存在潜在的协同调节作用。研究表明，LvBBX24 和 LvbZIP44 协同调控百合花青素积累。这些发现提供了令人信服的证据，支持 LvBBX24 和 LvbZIP44 可能在 LvMYB5 启动子区域周围形成一个环状螺旋以调节花青素生物合成的观点。