A noble metal-enhanced Au@CuO heterostructure with multienzyme-mimicking activities for colorimetric detection of tannic acid,Analyst

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A noble metal-enhanced Au@CuO heterostructure with multienzyme-mimicking activities for colorimetric detection of tannic acid
Analyst ( IF 3.6 ) Pub Date : 2024-07-31 , DOI: 10.1039/d4an01013b
Xin Kang _{1,

2} , Yiping Ren ₁ , Jin Wang ₁ , Xu Zhu ₁ , Ning Xin ₁ , Fenglei Gao ₁ , Dehong Yu _{1,

3}

Affiliation

Nanozymes, serving as synthetic alternatives to natural enzymes, offer several benefits including cost-effectiveness, enzyme-like catalytic abilities, enhanced stability, adjustable catalytic activity, easy recyclability, mild reaction conditions, and environmental friendliness. Nonetheless, the ongoing quest to develop nanozymes with enhanced activity and to delve into the catalytic mechanism remains a challenge. In our research, we effectively developed Au@CuO nanocomposites (Au@CuO Nc), replicating the functions of four enzymes found in nature: peroxidase (POD), catalase (CAT), glutathione peroxidase (GPx), and oxidase (OXD). The catalytic efficiency of Au@CuO Nc for TMB oxidation (oxTMB) was approximately 4.8 times greater than that of plain Cu₂O cubes, attributed to the synergistic catalytic impact between the Au element and Cu₂O within Au@CuO Nc. Mechanistic studies revealed that the novel Au@CuO Nc nanozyme greatly enhances the decomposition of H₂O₂ to reactive oxygen species (ROS) intermediates (˙OH, ˙O₂⁻ and ¹O₂), resulting in increased POD-like activity of the single-component Cu₂O cubes. When an antioxidant like TA was added to the chromogenic system, it converted oxTMB into a colorless form of TMB, enabling further evaluation of TA. Hence, a colorimetric sensor was developed for the rapid and precise quantitative measurement of TA, demonstrating strong linearity between 0.3 and 2.4 μM and featuring a low detection threshold of 0.25 μM. Moreover, this sensor was effectively utilized for the assessment of TA in actual tea samples. This work innovatively proposes a simplified and reliable strategy for the advanced design of highly effective Cu-based nanozymes, enhancing enzyme-like reactions for simultaneous, on-site colorimetric probing of antioxidants.

中文翻译：

具有多酶模拟活性的贵金属增强 Au@CuO 异质结构，用于比色检测单宁酸

纳米酶作为天然酶的合成替代品，具有多种优点，包括成本效益、类酶催化能力、增强的稳定性、可调节的催化活性、易于回收、反应条件温和和环境友好。尽管如此，开发具有增强活性的纳米酶并深入研究催化机制的持续探索仍然是一个挑战。在我们的研究中，我们有效地开发了Au@CuO纳米复合材料（Au@CuO Nc），复制了自然界中发现的四种酶的功能：过氧化物酶（POD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和氧化酶（OXD）。 Au@CuO Nc对TMB氧化(oxTMB)的催化效率比普通Cu ₂ O立方体高约4.8倍，这归因于Au@CuO Nc中Au元素和Cu ₂ O之间的协同催化作用。机理研究表明，新型Au@CuO Nc纳米酶极大地增强了H ₂ O ₂分解为活性氧（ROS）中间体（˙OH、˙O ₂ ⁻和¹ O ₂ ），从而增加了POD样活性单组分 Cu ₂ O 立方体。当将 TA 这样的抗氧化剂添加到显色系统中时，它会将 oxTMB 转化为无色形式的 TMB，从而能够进一步评估 TA。因此，我们开发了一种比色传感器，用于快速、精确地定量测量 TA，表现出 0.3 至 2 之间的强线性度。4 μM，检测阈值低至 0.25 μM。此外，该传感器被有效地用于实际茶叶样品中TA的评估。这项工作创新性地提出了一种简化且可靠的策略，用于高效铜基纳米酶的先进设计，增强酶样反应，以同时进行抗氧化剂的现场比色探测。

更新日期：2024-07-31

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