Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Construction of a DNA walker nanomachine aptasensor for simultaneous detection of dual-cancer biomarkers
Analyst ( IF 3.6 ) Pub Date : 2024-07-31 , DOI: 10.1039/d4an00865k Songbai Tian 1, 2 , Jiao Zheng 1, 3 , Xinghu Ji 3 , Fuxiang Zhou 1 , Zhike He 1, 3
Analyst ( IF 3.6 ) Pub Date : 2024-07-31 , DOI: 10.1039/d4an00865k Songbai Tian 1, 2 , Jiao Zheng 1, 3 , Xinghu Ji 3 , Fuxiang Zhou 1 , Zhike He 1, 3
Affiliation
|
While it is recognized that early diagnosis of cancer-related biomarkers can become an effective avenue for timely treatment and successfully improve patient survival, it remains challenging to get accurate inspection results. Currently, most reported cancer biomarker sensing methods are focused on the quantitative detection of a single type of biomarker, which makes accurate medical diagnostics difficult. In this work, we constructed a DNA walker nanomachine aptasensor based on gold nanoparticles for the simultaneous sensing of dual cancer biomarkers. The aptamers, labelled with a fluorophore, hybridized with complementary strands on the gold nanoparticle surface, serve as a walking track. Target analytes bind to their specific aptamers, leading to the dissociation of the unstable double-strand spherical nucleic acid. Exonuclease I (Exo I) selectively digested the aptamers bound with the target analytes, then the released targets go back to the next apamers on the gold nanopareticles surface for walking. The use of spherical nucleic acid probes improved the sensitivity of analyte detection. Exo I provided a driving power for target recycling and considerably improved the sensitivity of the aptasensor as well. The DNA walker nanomachine aptasensor was successfully applied for the detection of carcinoembryonic antigen (CEA) in the range of 0.167 to 3.34 ng mL−1, and mucin-1 (MUC-1) in the same range. Moreover, we used the two aptamers to construct the DNA walker nanomachine and achieved the simultaneous detection of CEA and MUC-1, thus having great potential for biomolecular logic gate construction and early disease diagnosis.
中文翻译：
用于同时检测双癌症生物标志物的DNA walker纳米机器适体传感器的构建
尽管人们认识到癌症相关生物标志物的早期诊断可以成为及时治疗并成功提高患者生存率的有效途径，但获得准确的检查结果仍然具有挑战性。目前，大多数报道的癌症生物标志物传感方法都集中于单一类型生物标志物的定量检测，这使得准确的医学诊断变得困难。在这项工作中，我们构建了一种基于金纳米粒子的 DNA walker 纳米机器适体传感器，用于同时传感双重癌症生物标志物。用荧光团标记的适体与金纳米粒子表面上的互补链杂交，充当行走轨迹。目标分析物与其特定适体结合，导致不稳定的双链球形核酸解离。核酸外切酶 I (Exo I) 选择性消化与目标分析物结合的适体，然后释放的目标物返回到金纳米颗粒表面上的下一个适体进行行走。球形核酸探针的使用提高了分析物检测的灵敏度。 Exo I 为目标回收提供了驱动力，并显着提高了适体传感器的灵敏度。 DNA walker纳米机器适体传感器成功应用于0.167至3.34 ng mL -1范围内的癌胚抗原（CEA）和相同范围内的粘蛋白-1（MUC-1）的检测。此外，我们利用这两种适配体构建了DNA walker纳米机器，实现了CEA和MUC-1的同时检测，在生物分子逻辑门构建和早期疾病诊断方面具有巨大潜力。
更新日期：2024-07-31
中文翻译：
用于同时检测双癌症生物标志物的DNA walker纳米机器适体传感器的构建
尽管人们认识到癌症相关生物标志物的早期诊断可以成为及时治疗并成功提高患者生存率的有效途径，但获得准确的检查结果仍然具有挑战性。目前，大多数报道的癌症生物标志物传感方法都集中于单一类型生物标志物的定量检测，这使得准确的医学诊断变得困难。在这项工作中，我们构建了一种基于金纳米粒子的 DNA walker 纳米机器适体传感器，用于同时传感双重癌症生物标志物。用荧光团标记的适体与金纳米粒子表面上的互补链杂交，充当行走轨迹。目标分析物与其特定适体结合，导致不稳定的双链球形核酸解离。核酸外切酶 I (Exo I) 选择性消化与目标分析物结合的适体，然后释放的目标物返回到金纳米颗粒表面上的下一个适体进行行走。球形核酸探针的使用提高了分析物检测的灵敏度。 Exo I 为目标回收提供了驱动力，并显着提高了适体传感器的灵敏度。 DNA walker纳米机器适体传感器成功应用于0.167至3.34 ng mL -1范围内的癌胚抗原（CEA）和相同范围内的粘蛋白-1（MUC-1）的检测。此外，我们利用这两种适配体构建了DNA walker纳米机器，实现了CEA和MUC-1的同时检测，在生物分子逻辑门构建和早期疾病诊断方面具有巨大潜力。
京公网安备 11010802027423号