Real‐time tracking of drug release from nanomedicine in vivo is crucial for optimizing its therapeutic efficacy in clinical settings, particularly in dosage control and determining the optimal therapeutic window. However, most current real‐time tracking systems require a tedious synthesis and purification process. Herein, a supramolecular nano‐tracker (SNT) capable of real‐time tracking of drug release in vivo based on non‐covalent host‐guest interactions is presented. By integrating multiple cavities into a single nanoparticle, SNT achieves co‐loading of drugs and probes while efficiently quenching the photophysical properties of the probe through host‐guest complexation. Moreover, SNT is readily degraded under hypoxic tumor tissues, leading to the simultaneous release of drugs and probes and the fluorescence recovery of probes. With this spatial and temporal consistency in drug loading and fluorescence quenching, as well as drug release and fluorescence recovery, SNT successfully achieves real‐time tracking of drug release in vivo (Pearson r = 0.9166, R2 = 0.8247). Furthermore, the released drugs can synergize effectively with fluorescent probes upon light irradiation, achieving potent chemo‐photodynamic combination therapy in 4T1‐bearing mice with a significantly improved survival rate (33%), providing a potential platform to significantly advance the development of nanomedicine and achieve optimal therapeutic effects in the clinic.

中文翻译：

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用于实时跟踪药物释放和高效联合治疗的超分子纳米跟踪器


实时跟踪体内纳米药物的药物释放对于优化其在临床环境中的治疗效果至关重要，特别是在剂量控制和确定最佳治疗窗方面。然而，当前大多数实时跟踪系统需要繁琐的合成和纯化过程。本文提出了一种超分子纳米跟踪器（SNT），能够基于非共价主客体相互作用实时跟踪体内药物释放。通过将多个空腔集成到单个纳米颗粒中，SNT 实现了药物和探针的共同负载，同时通过主客体络合有效地淬灭了探针的光物理特性。此外，SNT在缺氧的肿瘤组织下很容易降解，导致药物和探针的同时释放以及探针的荧光恢复。凭借药物加载和荧光猝灭以及药物释放和荧光恢复的空间和时间一致性，SNT 成功实现了体内药物释放的实时跟踪（Pearson r = 0.9166，右2 = 0.8247）。此外，所释放的药物在光照射下可以与荧光探针有效协同，在4T1荷瘤小鼠中实现有效的化学光动力联合治疗，显着提高存活率（33%），为显着推进纳米医学和纳米医学的发展提供了一个潜在的平台。在临床上达到最佳的治疗效果。