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Combination of an enzyme-linked immunosorbent assay and an indirect fluorescence assay for a nationwide sero-survey of Toxoplasma gondii infection in pig herds in Taiwan
Preventive Veterinary Medicine ( IF 2.2 ) Pub Date : 2024-07-14 , DOI: 10.1016/j.prevetmed.2024.106286 Kendy Tzu-Yun Teng , Chao-Chin Chang , Shao-Wen Hung , Ming-Tang Chiou , Chao-Nan Lin , Cheng-Yao Yang
Preventive Veterinary Medicine ( IF 2.2 ) Pub Date : 2024-07-14 , DOI: 10.1016/j.prevetmed.2024.106286 Kendy Tzu-Yun Teng , Chao-Chin Chang , Shao-Wen Hung , Ming-Tang Chiou , Chao-Nan Lin , Cheng-Yao Yang
is a zoonotic pathogen that can infect farm animals, companion animals, and humans, sometimes causing public health issues. In Taiwan, the pig industry is a vital agricultural industry, with a self-sufficiency rate of 91 %, and pigs are also food-producing animal reservoirs of . Infected pigs are usually asymptomatic, and abortions and death may occur in severe cases. We combined an enzyme-linked immunosorbent assay (ELISA) and an indirect fluorescence assay (IFA) to investigate the seroprevalence of among pig populations in Taiwan. A stratified sampling approach to determine the number of sample farms proportional to the number of pig farms in each county was employed, with 15 blood samples collected at each farm between July and September 2017. With the tested results, empirical Bayesian smoothing was utilized to assess the proportion of -positive farms at the county level. Bayesian mixed-effects logistic regression models, incorporating farm and county as random effects, were employed to investigate associations between test results and potential risk factors. A total of 930 serum samples from 62 pig farms were collected and tested. An overall herd prevalence of 27.4 % was shown with the seroprevalence in northern Taiwan being greater than that in southern Taiwan. The sampling month and companion dog density in 2017 were significantly associated with infections in pigs. With every increase in the number of companion dogs per km² at the county level, the odds of infection in pigs increased by 4.7 % (95 % CI: 1.7–8.9 %). This study demonstrated that combining ELISA for screening with IFA for confirmation is a cost-effective and time-saving method for conducting a large-scale sample investigation. This was also the first nationwide, cross-sectional study in Taiwanese pig herds to investigate infection.
更新日期：2024-07-14
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