The real-time screening of biomolecules and single cells in biochips is extremely important for disease prediction and diagnosis, cellular analysis, and life science research. Barcode biochip technology, which is integrated with microfluidics, typically comprises barcode array, sample loading, and reaction unit array chips. Here, we present a review of microfluidics barcode biochip analytical approaches for the high-throughput screening of biomolecules and single cells, including protein biomarkers, microRNA (miRNA), circulating tumor DNA (ctDNA), single-cell secreted proteins, single-cell exosomes, and cell interactions. We begin with an overview of current high-throughput detection and analysis approaches. Following this, we outline recent improvements in microfluidic devices for biomolecule and single-cell detection, highlighting the benefits and limitations of these devices. This paper focuses on the research and development of microfluidic barcode biochips, covering their self-assembly substrate materials and their specific applications with biomolecules and single cells. Looking forward, we explore the prospects and challenges of this technology, with the aim of contributing toward the use of microfluidic barcode detection biochips in medical diagnostics and therapies, and their large-scale commercialization.

中文翻译：

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用于高通量实时生物分子和单细胞筛选的微流控条码生物芯片


生物芯片中生物分子和单细胞的实时筛选对于疾病预测和诊断、细胞分析和生命科学研究极为重要。条码生物芯片技术与微流控集成，通常包括条码阵列、样品加载和反应单元阵列芯片。在这里，我们综述了用于生物分子和单细胞高通量筛选的微流控条形码生物芯片分析方法，包括蛋白质生物标志物、微小RNA (miRNA)、循环肿瘤DNA (ctDNA)、单细胞分泌蛋白、单细胞外泌体和细胞相互作用。我们首先概述当前的高通量检测和分析方法。接下来，我们概述了用于生物分子和单细胞检测的微流体装置的最新改进，强调了这些装置的优点和局限性。本文重点研究微流控条码生物芯片的研究和开发，涵盖其自组装基底材料及其在生物分子和单细胞中的具体应用。展望未来，我们探讨该技术的前景和挑战，旨在为微流控条码检测生物芯片在医疗诊断和治疗中的使用及其大规模商业化做出贡献。