Increasing prevalence of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) constitutes an emerging water safety issue globally. However, the effectiveness of current water treatment techniques in the control of ARGs dissemination remains controversial. Here, we develop a dual-zone strategy based on piezoelectric membrane filtration to efficiently eliminate waterborne antibiotic resistance. Following complete ARB inactivation and effective ARGs reduction in the retentate near the membrane surface (zone 1), a subsequent trans-membrane process (zone 2) further promotes ARGs elimination in the permeate, due to boosted interactions between ARGs and reactive oxygen species (ROS) generated from piezoelectric peroxymonosulfate activation and minimized ROS competition from inactivated ARB within piezocatalytic channels. The abundance of ARGs was largely reduced to ~1.0 × 10³ copies·mL⁻¹ in the permeate from ~5.0 × 10⁶ copies·mL⁻¹ in the feed solution. The singlet oxygen (¹O₂) is demonstrated to be primarily responsible for ARB inactivation, while ¹O₂, hydroxyl radical (•OH), sulfate radical (SO₄^·−) and superoxide radical (^·O₂⁻) can all participate in ARGs degradation. Our work demonstrates that the piezoelectric membrane-based dual-zone strategy has great potential to control the risk of ARGs dissemination.

抗生素抗性细菌（ARB）和抗生素抗性基因（ARG）的日益流行构成了全球新出现的水安全问题。然而，当前水处理技术在控制 ARGs 传播方面的有效性仍然存在争议。在这里，我们开发了一种基于压电膜过滤的双区策略，以有效消除水源抗生素耐药性。在膜表面（区域 1）附近的滞留物中 ARB 完全失活和 ARG 有效减少后，由于 ARG 和活性氧 (ROS) 之间增强的相互作用，随后的跨膜过程（区域 2）进一步促进渗透物中 ARG 的消除。 ）由压电过一硫酸盐活化产生，并最大限度地减少了压电催化通道内失活 ARB 的 ROS 竞争。渗透液中 ARG 的丰度从约 5.0 × 10 ⁶ 拷贝·mL ⁻¹ 拷贝·mL ⁻¹ 在进料溶液中。单线态氧 ( ¹ O ₂ ) 被证明是 ARB 失活的主要原因，而 ¹ O ₂ 、羟基自由基 ( •OH)、硫酸根(SO ₄ ^·− )和超氧自由基(O ₂ ⁻ )都可以参与ARGs的降解。我们的工作表明，基于压电膜的双区策略在控制 ARG 传播风险方面具有巨大潜力。