Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Continuous flow delivery system for the perfusion of scaffold-based 3D cultures
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-07-26 , DOI: 10.1039/d4lc00480a Zachary R Sitte 1 , Elizabeth E Karlsson 1 , Haolin Li 2 , Haibo Zhou 2, 3 , Matthew R Lockett 1, 4
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-07-26 , DOI: 10.1039/d4lc00480a Zachary R Sitte 1 , Elizabeth E Karlsson 1 , Haolin Li 2 , Haibo Zhou 2, 3 , Matthew R Lockett 1, 4
Affiliation
|
The paper-based culture platform developed by Whitesides readily incorporates tissue-like structures into laboratories with established workflows that rely on monolayer cultures. Cell-laden hydrogels are deposited in these porous scaffolds with micropipettes; these scaffolds support the thin gel slabs, allowing them to be evaluated individually or stacked into thick constructs. The paper-based culture platform has inspired many basic and translational studies, each exploring how readily accessible materials can generate complex structures that mimic aspects of tissues in vivo. Many of these examples have relied on static culture conditions, which result in diffusion-limited environments and cells experiencing pericellular hypoxia. Perfusion-based systems can alleviate pericellular hypoxia and other cell stresses by continually exposing the cells to fresh medium. These perfusion systems are common in microfluidic and organ-on-chip devices supporting cells as monolayer cultures or as 3D constructs. Here, we introduce a continuous flow delivery system, which uses parts readily produced with 3D printing to provide a self-contained culture platform in which cells in paper or other scaffolds are exposed to fresh (flowing) medium. We demonstrate the utility of this device with examples of cells maintained in single cell-laden scaffolds, stacks of cell-laden scaffolds, and scaffolds that contain monolayers of endothelial cells. These demonstrations highlight some possible experimental questions that can be enabled with readily accessible culture materials and a perfusion-based device that can be readily fabricated.
中文翻译：
用于灌注基于支架的 3D 培养物的连续流输送系统
Whitesides 开发的纸质培养平台可以轻松地将组织样结构纳入实验室，并建立依赖于单层培养的工作流程。使用微量移液器将充满细胞的水凝胶沉积在这些多孔支架中；这些支架支撑着薄的凝胶板，使它们能够单独评估或堆叠成厚的结构。基于纸质的培养平台激发了许多基础和转化研究，每项研究都探索易于获取的材料如何生成模仿体内组织各个方面的复杂结构。这些例子中的许多都依赖于静态培养条件，这导致扩散受限的环境和细胞经历细胞周缺氧。基于灌注的系统可以通过不断地将细胞暴露于新鲜培养基来缓解细胞周缺氧和其他细胞应激。这些灌注系统常见于支持细胞作为单层培养物或 3D 构建体的微流体和器官芯片设备中。在这里，我们介绍了一种连续流输送系统，该系统使用通过 3D 打印轻松生产的部件来提供一个独立的培养平台，其中纸或其他支架中的细胞暴露于新鲜（流动）培养基。我们通过维持在单细胞负载支架、细胞负载支架堆叠和包含单层内皮细胞的支架中的细胞示例来展示该装置的实用性。这些演示强调了一些可能的实验问题，这些问题可以通过易于获取的培养材料和易于制造的基于灌注的设备来实现。
更新日期：2024-07-26
中文翻译：
用于灌注基于支架的 3D 培养物的连续流输送系统
Whitesides 开发的纸质培养平台可以轻松地将组织样结构纳入实验室，并建立依赖于单层培养的工作流程。使用微量移液器将充满细胞的水凝胶沉积在这些多孔支架中；这些支架支撑着薄的凝胶板，使它们能够单独评估或堆叠成厚的结构。基于纸质的培养平台激发了许多基础和转化研究，每项研究都探索易于获取的材料如何生成模仿体内组织各个方面的复杂结构。这些例子中的许多都依赖于静态培养条件，这导致扩散受限的环境和细胞经历细胞周缺氧。基于灌注的系统可以通过不断地将细胞暴露于新鲜培养基来缓解细胞周缺氧和其他细胞应激。这些灌注系统常见于支持细胞作为单层培养物或 3D 构建体的微流体和器官芯片设备中。在这里，我们介绍了一种连续流输送系统，该系统使用通过 3D 打印轻松生产的部件来提供一个独立的培养平台，其中纸或其他支架中的细胞暴露于新鲜（流动）培养基。我们通过维持在单细胞负载支架、细胞负载支架堆叠和包含单层内皮细胞的支架中的细胞示例来展示该装置的实用性。这些演示强调了一些可能的实验问题，这些问题可以通过易于获取的培养材料和易于制造的基于灌注的设备来实现。
京公网安备 11010802027423号