IntroductionCalcein was used to develop a shell marking method for Potamocorbula laevis.MethodsThe suitable conditions for marking were investigated, including marking concentration, immersion time, and water temperature. The impacts and feasibility of the marking method were assessed based on the survival rate of P. laevis, the success rate of fluorescence marking, marking quality, and alterations in activities of antioxidant enzymes in the digestive gland of the experimental bivalves. Two concentrations of calcein (20 and 50 mg/L) were used and the immersion time included 1 and 2 h, respectively. The experiment was performed in two rounds, with water temperatures of 12.84 ± 0.09 and 24.18 ± 0.04 °C, respectively.Results and discussionThe results indicated that calcein did not significantly impact the survival of P. laevis after 7 d of recovery. The catalase activity and malondialdehyde content in low temperature-marked P. laevis showed significant decreases, and the relative abundances of certain fatty acids also exhibited significant changes within 2 h post exposure to 20 mg/L of calcein. However, these indicators returned to normal levels within 7 d. The marking impact of calcein was proportional to the calcein concentration and immersion time. Higher temperature generated a negative impact on the marking effect of 20 mg/L of calcein, while no obvious impacts were observed for 50 mg/L of calcein. The marking success rates and the recapture rates of P. laevis for in situ tests in the two experimental groups were both 100% and 4.44 ± 1.29% after one month. Also, the recapture marking rates and the marking good rates of the recaptured individuals were both 100%. There were no significant differences between these parameters for 50 and 75 mg/L of calcein. Given the cost and safety of labeling, a strategy in terms of an immersion in 50 mg/L of calcein for 2 h could be considered as an effective in situ labeling scheme for P. laevis. In conclusion, calcein can be employed as a marking method for P. laevis. These findings could be potentially beneficial for development of in situ labeling technology, proliferation as well as release of shellfish in tidal flats and resource conservation.

中文翻译：

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钙黄绿素作为顺眼子藻化学标记物的功效


介绍使用钙黄绿素开发了一种壳标记方法眼子藻方法探讨适合标记的条件，包括标记浓度、浸泡时间、水温。根据存活率评估了标记方法的影响和可行性。非洲对虾、荧光标记的成功率、标记质量以及实验双壳类消化腺抗氧化酶活性的变化。使用两种浓度的钙黄绿素（20 和 50 mg/L），浸泡时间分别为 1 和 2 小时。实验分两轮进行，水温分别为12.84±0.09和24.18±0.04℃。结果与讨论结果表明，钙黄绿素对存活率没有显着影响。非洲对虾恢复7天后。低温标记过氧化氢酶活性和丙二醛含量非洲对虾暴露于 20 mg/L 钙黄绿素后 2 小时内，某些脂肪酸的相对丰度也表现出显着的变化。但这些指标在7天内就恢复到正常水平。钙黄绿素的标记影响与钙黄绿素浓度和浸泡时间成正比。较高的温度对20mg/L的钙黄绿素的标记效果产生负面影响，而50mg/L的钙黄绿素则没有观察到明显的影响。标记成功率和重新捕获率非洲对虾为了就地1个月后，两个实验组的测试结果均为100%和4.44±1.29%。 重新捕获个体的重新标记率和标记良好率均为100%。对于 50 和 75 mg/L 的钙黄绿素，这些参数之间没有显着差异。考虑到标签的成本和安全性，在 50 mg/L 钙黄绿素中浸泡 2 小时的策略可被认为是一种有效的策略。就地标签计划非洲对虾。总之，钙黄绿素可以作为标记方法非洲对虾。这些发现可能有利于发展就地标签技术、滩涂贝类的增殖和释放以及资源保护。