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Integrating QTL mapping and transcriptomics to decipher the genetic architecture of sterol metabolism in Brassica napus L
Horticulture Research ( IF 7.6 ) Pub Date : 2024-07-24 , DOI: 10.1093/hr/uhae196
Yiyi Xiong 1 , Guangyuan Lu 2 , Huaixin Li 1 , Jianjie He 1 , Shipeng Fan 1 , Shuxiang Yan 1 , Liangxiao Zhang 3 , Haibo Jia 1 , Maoteng Li 1
Horticulture Research ( IF 7.6 ) Pub Date : 2024-07-24 , DOI: 10.1093/hr/uhae196
Yiyi Xiong 1 , Guangyuan Lu 2 , Huaixin Li 1 , Jianjie He 1 , Shipeng Fan 1 , Shuxiang Yan 1 , Liangxiao Zhang 3 , Haibo Jia 1 , Maoteng Li 1
Affiliation
Sterols are secondary metabolites commonly found in rapeseed that play crucial physiological roles in plants and also benefit human health. Consequently, unraveling the genetic basis of sterol synthesis in rapeseed is highly important. In this study, 21 individual sterols as well as total sterol (TS) content were detected in a double haploid (DH) population of Brassica napus, and a total of 24 quantitative trait loci (QTL) and 157 mQTL were identified that were associated with TS and different individual sterols. Time-series transcriptomic analysis showed that the differentially expressed genes (DEGs) involved in sterol and lipid biosynthesis pathways were enriched. Additionally, a regulatory network between sterol-related DEGs and transcription factors (TFs) was established using coexpression analysis. Some candidate genes were identified with the integration of transcriptomic analysis and QTL mapping, and the key candidate gene BnSQS1.C03 was selected for further functional analysis. BnSQS1.C03 demonstrated squalene synthase activity in vitro and increased the TS by 3.8% when overexpressed in Arabidopsis. The present results provide new insights into sterol regulatory pathways and a valuable genetic basis for breeding rapeseed varieties with high sterol content in the future.
中文翻译:
整合 QTL 定位和转录组学破译甘蓝型油菜 L 甾醇代谢的遗传结构
甾醇是油菜籽中常见的次生代谢产物,在植物中起着至关重要的生理作用,也有益于人体健康。因此,揭示油菜籽中甾醇合成的遗传基础非常重要。在本研究中,在甘蓝型油菜的双单倍体 (DH) 群体中检测到 21 种甾醇和总甾醇 (TS) 含量,共鉴定出 24 个与 TS 和不同甾醇相关的数量性状位点 (QTL) 和 157 个 mQTL。时间序列转录组学分析显示,参与甾醇和脂质生物合成途径的差异表达基因 (DEGs) 富集。此外,使用共表达分析建立了甾醇相关 DEGs 和转录因子 (TFs) 之间的调控网络。通过转录组学分析和 QTL 定位的整合鉴定了一些候选基因,并筛选出关键候选基因 BnSQS1.C03 进行进一步的功能分析。BnSQS1.C03 在体外表现出角鲨烯合酶活性,并且在拟南芥中过表达时,TS 增加 3.8%。目前的结果为甾醇调控途径提供了新的见解,并为未来培育高甾醇含量的油菜籽品种提供了宝贵的遗传基础。
更新日期:2024-07-24
中文翻译:
整合 QTL 定位和转录组学破译甘蓝型油菜 L 甾醇代谢的遗传结构
甾醇是油菜籽中常见的次生代谢产物,在植物中起着至关重要的生理作用,也有益于人体健康。因此,揭示油菜籽中甾醇合成的遗传基础非常重要。在本研究中,在甘蓝型油菜的双单倍体 (DH) 群体中检测到 21 种甾醇和总甾醇 (TS) 含量,共鉴定出 24 个与 TS 和不同甾醇相关的数量性状位点 (QTL) 和 157 个 mQTL。时间序列转录组学分析显示,参与甾醇和脂质生物合成途径的差异表达基因 (DEGs) 富集。此外,使用共表达分析建立了甾醇相关 DEGs 和转录因子 (TFs) 之间的调控网络。通过转录组学分析和 QTL 定位的整合鉴定了一些候选基因,并筛选出关键候选基因 BnSQS1.C03 进行进一步的功能分析。BnSQS1.C03 在体外表现出角鲨烯合酶活性,并且在拟南芥中过表达时,TS 增加 3.8%。目前的结果为甾醇调控途径提供了新的见解,并为未来培育高甾醇含量的油菜籽品种提供了宝贵的遗传基础。