Naive T cells recirculate between the spleen and lymph nodes where they mount immune responses when meeting dendritic cells presenting foreign antigen. As this may happen anywhere, naive T cells ought to visit all lymph nodes. Here, deep sequencing almost-complete TCR repertoires led to a comparison of different lymph nodes within and between individual mice. We find strong evidence for a deterministic CD4/CD8 lineage choice and a consistent spatial structure. Specifically, some T cells show a preference for one or multiple lymph nodes, suggesting that their TCR interacts with locally presented (self-)peptides. These findings are mirrored in TCR-transgenic mice showing localized CD69 expression, retention, and cell division. Thus, naive T cells intermittently sense antigenically dissimilar niches, which is expected to affect their homeostatic competition.

中文翻译：

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TCR 将初始 T 细胞分配至首选淋巴结


初始 T 细胞在脾脏和淋巴结之间循环，当遇到呈递外来抗原的树突状细胞时，它们会产生免疫反应。由于这种情况可能发生在任何地方，因此初始 T 细胞应该访问所有淋巴结。在这里，对几乎完整的 TCR 库进行深度测序，从而对小鼠体内和小鼠之间的不同淋巴结进行了比较。我们发现了确定性 CD4/CD8 谱系选择和一致的空间结构的有力证据。具体来说，一些 T 细胞表现出对一个或多个淋巴结的偏好，表明它们的 TCR 与局部存在的（自身）肽相互作用。这些发现在 TCR 转基因小鼠中得到了反映，显示出局部 CD69 表达、保留和细胞分裂。因此，幼稚 T 细胞间歇性地感知抗原不同的生态位，这预计会影响它们的稳态竞争。