In Vivo Detection of Abscisic Acid in Tomato Leaves Based on a Disposable Stainless Steel Electrochemical Immunosensor,Journal of Agricultural and Food Chemistry

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In Vivo Detection of Abscisic Acid in Tomato Leaves Based on a Disposable Stainless Steel Electrochemical Immunosensor
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-07-25 , DOI: 10.1021/acs.jafc.4c03594
Lingjuan Tang _{1,

2} , Zhiyao Zhang ₁ , Ling Sun ₁ , Xu Gao ₃ , Xinyue Zhao ₁ , Xinru Chen ₁ , Xingyu Zhu ₁ , Aixue Li ₄ , Lijun Sun ₁

Affiliation

Abscisic acid (ABA) plays an important regulatory role in plants. It is very critical to obtain the dynamic changes of ABA in situ for botanical research. Herein, coupled with paper-based analysis devices, electrochemical immunoelectrodes based on disposable stainless steels sheet were developed for ABA detection in plants in situ. The stainless steel sheets were modified with carbon cement, ferrocene-graphene oxide-multi walled carbon nanotubes nanocomposites, and ABA antibodies. The system can detect the ABA in the range of 1 nM to 100 μM, with a limit of detection of 100 pM. The ABA content in tomato leaves under high salinity was detected in situ. The trend of ABA changes was similar to the expression of SlNCED1 and SlNCED2. Overall, this study offers an approach for in situ detection of ABA in plants, which will help to study the regulation mechanism of ABA in plants and to promote the development of precision agriculture.

中文翻译：

基于一次性不锈钢电化学免疫传感器的番茄叶中脱落酸的体内检测

脱落酸（ABA）在植物中起着重要的调节作用。原位获取ABA的动态变化对于植物学研究非常关键。在此，结合纸基分析装置，开发了基于一次性不锈钢片的电化学免疫电极，用于植物中的ABA原位检测。不锈钢板采用碳水泥、二茂铁-氧化石墨烯-多壁碳纳米管纳米复合材料和 ABA 抗体进行改性。该系统可以检测1 nM至100 μM范围内的ABA，检测限为100 pM。原位检测高盐度下番茄叶片ABA含量。 ABA的变化趋势与SlNCED1和SlNCED2的表达相似。总体而言，本研究为植物中ABA的原位检测提供了一种方法，有助于研究植物中ABA的调控机制，促进精准农业的发展。

更新日期：2024-07-25

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