Microscopic and Transcriptomic Analyses to Elucidate Antifungal Mechanisms of Bacillus velezensis TCS001 Lipopeptides against Botrytis cinerea,Journal of Agricultural and Food Chemistry

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Microscopic and Transcriptomic Analyses to Elucidate Antifungal Mechanisms of Bacillus velezensis TCS001 Lipopeptides against Botrytis cinerea
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-07-23 , DOI: 10.1021/acs.jafc.4c03323
Jing Jin _{1,

2} , Ran-Di Yang ₁ , Hao Cao ₁ , Ge-Ning Song ₁ , Feng Cui ₁ , Sha Zhou ₁ , Jing Yuan ₁ , Huan Qi ₃ , Ji-Dong Wang ₃ , Jie Chen ₁

Affiliation

Botrytis cinerea is an important fungal pathogen that causes gray mold disease in plants. Previously, Bacillus velezensis TCS001 live culture presented broad-spectrum antifungal activity against various plant pathogenic fungi and oomycetes, particularly B. cinerea. Here, the bioactivity of lipopeptides produced by TCS001 against B. cinerea was investigated. The IC₅₀ values of the crude lipopeptide extract (CLE) from TCS001 to suppress mycelial growth and conidial germination were 14.20 and 49.39 mg/L, respectively. SEM and TEM imaging revealed that CLE caused morphological deformities and ultrastructural changes in the mycelium. Transcriptomic analyses combined with ΔBcpsd mutant construction demonstrated that the CLE could confer antifungal activity via suppressing Bcpsd expression in the pathogen. In addition, the CLE activated the plant immune system by increasing the content of defense-related enzymes and the expression of marker genes in immunity signaling pathways in cucumber plants. Therefore, TCS001 CLE could be potentially developed into biopesticides for the biocontrol of gray mold disease.

中文翻译：

显微镜和转录组分析阐明贝莱斯芽孢杆菌 TCS001 脂肽抗灰葡萄孢的抗真菌机制

灰葡萄孢(Botrytis cinerea)是引起植物灰霉病的重要真菌病原体。此前，贝莱斯芽孢杆菌TCS001 活培养物对多种植物病原真菌和卵菌，特别是灰霉病菌，表现出广谱抗真菌活性。在此，研究了 TCS001 产生的脂肽针对灰霉病菌的生物活性。 TCS001 的粗脂肽提取物 (CLE) 抑制菌丝生长和分生孢子萌发的 IC ₅₀值分别为 14.20 和 49.39 mg/L。 SEM 和 TEM 成像显示 CLE 引起菌丝体形态畸形和超微结构变化。转录组分析与 Δ Bcpsd突变体构建相结合表明，CLE 可以通过抑制病原体中的Bcpsd表达来赋予抗真菌活性。此外，CLE通过增加黄瓜植物中防御相关酶的含量和免疫信号通路中标记基因的表达来激活植物免疫系统。因此，TCS001 CLE 有可能开发成生物农药，用于灰霉病的生物防治。

更新日期：2024-07-23

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