Background: Dandouchi polypeptide (DDCP) is derived from Semen Sojae Praeparatum (Dandouchi in Chinese), a fermented product of Glycine max (L.) Merr. Semen Sojae Praeparatum is widely used in the food industry for its unique flavor and nutritional value, and DDCP, as its derivative, also shows potential health benefits in food applications. However, the specific active substances responsible for Semen Sojae Praeparatum and the underlying mechanisms involved have not been fully elucidated. Methods: DDCP was extracted from Semen Sojae Praeparatum using enzymes, and its antidepressant effects were tested in chronic unpredictable mild stress (CUMS)-induced mice. Immunohistochemistry, immunofluorescence, and western blotting were used to analyze neurogenesis and the nuclear factor κB (NF-κB) pathway. Moreover, an adeno-associated virus (AAV) shRNA was used to induce tripartite motif-containing 67 (TRIM67) deficiency to examine the function of TRIM67 in the neuroprotective effects of DDCP in depressive disorders. Results: DDCP reduced depressive behaviors in CUMS mice and the expression of proinflammatory markers in the hippocampus. DDCP promoted neurogenesis and modulated the TRIM67/NF-κB pathway, with TRIM67 deficiency impairing its antidepressant effect. Conclusions: This research revealed that DDCP has a protective effect on countering depression triggered by CUMS. Notably, TRIM67 plays a crucial role in mitigating depression through DDCP, positioning DDCP as a potential therapeutic option for treating depressive disorders.

中文翻译：

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Dandouchi 多肽通过激活 CUMS 诱导小鼠的 TRIM67/NF-κB 通路减轻抑郁样行为并促进海马神经发生


背景：丹豆内多肽（DDCP）来源于大豆（Glycine max (L.) Merr.）的发酵产物。黄豆因其独特的风味和营养价值而被广泛应用于食品工业，而DDCP作为其衍生物，在食品应用中也显示出潜在的健康益处。然而，黄豆的具体活性物质及其相关机制尚未完全阐明。方法：利用酶从大豆中提取DDCP，并在慢性不可预测性轻度应激（CUMS）诱导的小鼠中测试其抗抑郁作用。使用免疫组织化学、免疫荧光和蛋白质印迹分析神经发生和核因子 κB (NF-κB) 通路。此外，使用腺相关病毒 (AAV) shRNA 诱导含有三重基序的 67 (TRIM67) 缺陷，以检查 TRIM67 在 DDCP 对抑郁症的神经保护作用中的功能。结果：DDCP 减少了 CUMS 小鼠的抑郁行为和海马促炎标记物的表达。 DDCP 促进神经发生并调节 TRIM67/NF-κB 通路，TRIM67 缺乏会损害其抗抑郁作用。结论：本研究表明 DDCP 对对抗 CUMS 引发的抑郁症具有保护作用。值得注意的是，TRIM67 在通过 DDCP 缓解抑郁症方面发挥着至关重要的作用，使 DDCP 成为治疗抑郁症的潜在治疗选择。