The symbiotic nitrogen fixation (SNF) of legumes is essential for their growth and development. However, the effects of SNF on the production of specific compounds in medicinal legumes remains unclear. In this study, we identified 364 differentially accumulated metabolites (DAMs) in roots triggered by SNF compared to non-inoculated control, including compounds like 6,7-Dihydroxy-4’-methoxy isoflavone, Puerarin, Biochanin A, Ginkgetin, Sieboldin, and Prunetin. A comparative transcriptomic analysis of nodulated roots and non-inoculated control revealed 9510 differentially expressed genes (DEGs). Integrating metabolomic and transcriptomic analysis of these DEGs and DAMs highlighted 37 genes associated with isoflavonoid biosynthesis. Notably, several key regulators in isoflavonoids biosynthesis were identified and confirmed by qRT-PCR. Overexpression of in roots led to a significantly increase of the enzyme activity of flavonoids biosynthesis and flavonoid contents, including Genistin, Daidzin, ononin, Luteolin, Luteoloside, and Apigenin. These findings shed light on effects of SNF on the bioactive compounds in medicinal legumes, and offer valuable insights for reducing the amount of chemical fertilizer use and promoting efficient cultivation of medicinal legumes.

中文翻译：

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综合代谢组学和转录组学辅助鉴定结节苦参 SfIF7GT 以提高类黄酮含量


豆科植物的共生固氮（SNF）对其生长和发育至关重要。然而，SNF 对药用豆类中特定化合物产生的影响仍不清楚。在这项研究中，我们鉴定了与未接种对照相比，由 SNF 引发的根部中 364 种差异积累的代谢物 (DAM)，包括 6,7-二羟基-4'-甲氧基异黄酮、葛根素、生物鸡素 A、银杏素、西博丁等化合物，以及普鲁内丁。对结瘤根和未接种对照的比较转录组分析揭示了 9510 个差异表达基因 (DEG)。对这些 DEG 和 DAM 进行整合代谢组学和转录组学分析，突出显示了与异黄酮类生物合成相关的 37 个基因。值得注意的是，通过 qRT-PCR 鉴定并证实了异黄酮生物合成中的几个关键调节因子。根部过表达导致类黄酮生物合成酶活性和类黄酮含量显着增加，包括染料木苷、大豆苷、ononin、木犀草素、木犀草苷和芹菜素。这些研究结果揭示了SNF对药用豆科植物生物活性化合物的影响，为减少化肥使用量、促进药用豆科植物高效种植提供了宝贵的见解。