Exogenous electrical stimulation has attracted considerable attention due to the advantages of microelectric induction and subsequent biological effects such as actin reorganization and reactive oxygen species (ROS) generation. Herein, an injectable hydrogel of BPR-ARG@Gel (BAG) with pyroelectric BPR nanoparticle loading and l-arginine (ARG) introduction was fabricated for advanced cancer therapy in vivo. Due to the photothermal effect, the holes and electrons in BPR nanoparticles were separated to produce an open-circuit voltage and consequently catalyze water H₂O to generate toxic superoxide (^•O₂^–) and hydroxyl radicals (^•OH). These ROS substances further oxidize ARG to produce NO for synergistic tumor treatments. The mice experiments indicated that the employment of BAG hydrogel incorporation with a near-infrared laser downregulated the heat shock protein and recruited immune cells with 5-fold-enhanced expression of proinflammatory cytokines of interferon-γ. It was also noteworthy that the injectable hydrogel of BAG substantially induced the generation of reactive oxygen/nitrogen species (ROS/RNS) with reliable biosafety and strong tumor inhibition. Overall, these findings have provided potentially new inspirations and a feasible strategy to translate this multifunctional hydrogel toward tumor therapy in a pyroelectric stimulation manner.

中文翻译：

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近红外介导的热释电催化通过可注射水凝胶实现持续的 ROS/RNS 生成和体内先进癌症治疗


由于微电感应的优点以及随后的肌动蛋白重组和活性氧（ROS）产生等生物效应，外源性电刺激引起了人们的广泛关注。在此，制备了一种可注射的 BPR-ARG@Gel (BAG) 水凝胶，其负载有热电 BPR 纳米颗粒并引入了 L-精氨酸 (ARG)，用于体内先进的癌症治疗。由于光热效应，BPR纳米颗粒中的空穴和电子分离，产生开路电压，从而催化水 H ₂ O 生成有毒的超氧化物（ ^• O ₂ ^– ）和羟基自由基（ ^• OH）。这些ROS物质进一步氧化ARG产生NO用于协同肿瘤治疗。小鼠实验表明，使用 BAG 水凝胶与近红外激光结合可下调热休克蛋白，并募集免疫细胞，干扰素-γ 促炎细胞因子的表达增强 5 倍。值得注意的是，BAG的可注射水凝胶显着诱导活性氧/氮物种（ROS/RNS）的产生，具有可靠的生物安全性和强的肿瘤抑制作用。总的来说，这些发现为将这种多功能水凝胶以热释电刺激方式转化为肿瘤治疗提供了潜在的新灵感和可行的策略。