Monitoring of the introduction of unapproved genetically modified (GM) events is required because the approval status of a GM event may differ from country to country. The on-site methods such as loop-mediated isothermal amplification (LAMP) offer a technological answer for the rapid GM detection beyond the laboratories. Real-time LAMP assays detecting one GM target were reported earlier. To increase the efficiency of the assay, a multiplex real-time LAMP simultaneously targeting Figwort Mosaic Virus promoter (P-FMV) that constructs region between the Cauliflower Mosaic Virus 35S promoter and cry1Ac gene (p35S-cry1Ac) and neomycin phosphotransferase II (nptII) marker gene was developed. The assay could detect as low as 0.1% for each GM target within 45 min. To the best of our knowledge, multiplexing in real-time LAMP using the Genie II system with applicability in GM detection has been reported herein for the first time. The developed method provides rapid, on-site, and real-time GM detection in seeds and food products.

中文翻译：

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基于退火曲线分析的多重实时环介导等温扩增 (LAMP)：种子和食品中转基因序列的现场多重检测


需要对未经批准的转基因 (GM) 事件的引入进行监测，因为转基因事件的批准状态可能因国家而异。环介导等温扩增 (LAMP) 等现场方法为实验室之外的快速转基因检测提供了技术答案。早期报道了检测一种 GM 靶点的实时 LAMP 检测。为了提高检测效率，多重实时 LAMP 同时靶向玄参花叶病毒启动子 (P-FMV)，在花椰菜花叶病毒 35S 启动子和cry1Ac 基因 (p35S-cry1Ac) 和新霉素磷酸转移酶 II (nptII) 之间构建区域开发了标记基因。该检测可在 45 分钟内检测出低至 0.1% 的每个 GM 靶点。据我们所知，本文首次报道了使用 Genie II 系统进行实时 LAMP 多重检测并适用于 GM 检测。所开发的方法可对种子和食品进行快速、现场、实时的转基因检测。