Rapid Screening of Biomarkers in KYSE-150 Cells Exposed to Polycyclic Aromatic Hydrocarbons via Inkjet Printing Single-Cell Mass Spectrometry,Analytical Chemistry

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Rapid Screening of Biomarkers in KYSE-150 Cells Exposed to Polycyclic Aromatic Hydrocarbons via Inkjet Printing Single-Cell Mass Spectrometry
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-07-25 , DOI: 10.1021/acs.analchem.4c02332
Ai Ren _{1,

2} , Fengming Chen _{1,

2} , Chenjie Ren _{1,

2} , Minli Yang _{1,

2} , Chang Wang _{1,

2} , Xuesong Feng ₃ , Feng Zhang ₁

Affiliation

Single-cell analysis by mass spectrometry (MS) is emerging as a powerful tool that not only contributes to cellular heterogeneity but also offers an unprecedented opportunity to predict pathology onset and facilitates novel biomarker discovery. However, the development of single-cell MS analysis techniques with a focus on sample extraction, separation, and ionization methods for volume-limited samples and complexity of cellular samples are still a big challenge. In this study, we present a high-throughput approach to inkjet drop on demand printing single-cell MS for rapid screening of biomarkers of polycyclic aromatic hydrocarbon (PAH) exposure at the KYSE-150 cell, aiming to elucidate the pathogenesis of PAH-induced esophageal cancer. With an analytical bulk KYSE-150 cell throughput of up to 51 cells per minute, the method provides a new opportunity for simultaneous single-cell analysis of multiple biomarkers. We screened 930 characteristic ions from 3,683 detected peak signals and identified 91 distinctive molecules that exhibited significant differences under various concentrations of PAH exposure. These molecules have potential as clinical diagnostic biomarkers. Additionally, the current study identifies specific biomarkers that behave completely opposite in single-cell and multicell lipidomics as the concentration of PAH changes. These biomarkers potentially subdivide KYSE-150 cells into PAH-sensitive and PAH-insensitive types, providing a basis for revealing PAH toxicity and disease pathogenesis from the heterogeneity of cellular metabolism.

中文翻译：

通过喷墨打印单细胞质谱快速筛选暴露于多环芳烃的 KYSE-150 细胞中的生物标志物

质谱（MS）单细胞分析正在成为一种强大的工具，不仅有助于细胞异质性，而且还提供了前所未有的机会来预测病理发生并促进新型生物标志物的发现。然而，针对体积有限的样品和细胞样品的复杂性，以样品提取、分离和电离方法为重点的单细胞质谱分析技术的发展仍然是一个巨大的挑战。在这项研究中，我们提出了一种高通量喷墨按需打印单细胞 MS 方法，用于快速筛选 KYSE-150 细胞中多环芳烃 (PAH) 暴露的生物标志物，旨在阐明 PAH 诱导的发病机制。食道癌。该方法的 KYSE-150 细胞分析通量高达每分钟 51 个细胞，为同时进行多个生物标志物的单细胞分析提供了新的机会。我们从 3,683 个检测到的峰值信号中筛选了 930 个特征离子，并鉴定了 91 个独特分子，这些分子在不同浓度的 PAH 暴露下表现出显着差异。这些分子具有作为临床诊断生物标志物的潜力。此外，当前的研究还确定了随着 PAH 浓度变化而在单细胞和多细胞脂质组学中表现完全相反的特定生物标志物。这些生物标志物有可能将KYSE-150细胞细分为PAH敏感型和PAH不敏感型，为从细胞代谢的异质性揭示PAH毒性和疾病发病机制提供了基础。

更新日期：2024-07-25

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