3D cellular self-assembly on optical disc-imprinted nanopatterns,Lab on a Chip

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3D cellular self-assembly on optical disc-imprinted nanopatterns
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-07-23 , DOI: 10.1039/d4lc00386a
Jeeyeon Lee ₁ , Chwee Teck Lim _{1,

2,

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Affiliation

Three-dimensional (3D) cellular assemblies, such as cancer spheroids and organoids, are increasingly valued for their physiological relevance, and versatility in biological applications. Nanopatterns that mimic the extracellular matrix provide crucial topological cues, creating a physiologically relevant cellular environment and guiding cellular behaviors. However, the high cost and complex, time-consuming nature of the nanofabrication process have limited the widespread adoption of nanopatterns in diverse biological applications. In this study, we present a straightforward and cost-effective elastomer replica molding method utilizing commercially available optical discs to generate various nanopatterns, such as nanogroove/ridge, nanoposts, and nanopits, varying in spacing and heights. Using the nanopatterned well chips (NW-Chips), we demonstrated the efficient formation of 3D multicellular self-assemblies of three different types of cancer cells. Our findings highlight the accessibility and affordability of optical discs as tools for nanopattern generation, offering promising avenues for modulating cell behaviors and advancing diverse biological applications.

中文翻译：

光盘压印纳米图案上的 3D 细胞自组装

三维 (3D) 细胞组装体，例如癌症球体和类器官，因其生理相关性和生物应用的多功能性而越来越受到重视。模仿细胞外基质的纳米图案提供了重要的拓扑线索，创造了生理相关的细胞环境并指导细胞行为。然而，纳米制造过程的高成本和复杂、耗时的性质限制了纳米图案在各种生物应用中的广泛采用。在这项研究中，我们提出了一种简单且经济有效的弹性体复制成型方法，利用市售光盘来生成各种纳米图案，例如纳米槽/脊、纳米柱和纳米坑，其间距和高度各不相同。使用纳米图案孔芯片（NW-Chips），我们证明了三种不同类型癌细胞的 3D 多细胞自组装的有效形成。我们的研究结果强调了光盘作为纳米图案生成工具的可及性和可承受性，为调节细胞行为和推进多样化的生物应用提供了有前景的途径。

更新日期：2024-07-23

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