Surface modification could enhance the cell internalization efficiency of nanovehicles for targeted gene or drug delivery. However, the influence of surface modification parameters, including recognition manners, valences, and patterns, is often clouded, especially for the endocytosis of DNA nanostructures in customized shapes. Focusing on an icosahedral DNA framework, we systematically programmed three distinct types of ligands with diverse valence and spatial distribution on their outer surface to study the internalization efficiency, endocytic pathways, and postinternalization fate. The comparison in different aspects of parameters deepens our understanding of the intricate relationship between surface modification and cell entry behavior, offering insights crucial for designing and optimizing DNA framework nanostructures for potent cell-targeted purposes.

中文翻译：

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通过可编程表面修饰调节二十面体 DNA 框架的细胞内化行为


表面修饰可以提高纳米载体用于靶向基因或药物输送的细胞内化效率。然而，表面修饰参数（包括识别方式、化合价和模式）的影响常常被掩盖，特别是对于定制形状的 DNA 纳米结构的内吞作用。围绕二十面体DNA框架，我们系统地编程了三种不同类型的配体，其外表面具有不同的化合价和空间分布，以研究内化效率、内吞途径和内化后命运。参数不同方面的比较加深了我们对表面修饰和细胞进入行为之间复杂关系的理解，为设计和优化 DNA 框架纳米结构以实现有效的细胞靶向目的提供了至关重要的见解。