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Lipolysis and Sterol Stability and Bioaccessibility of Wholemeal Rye Bread Enriched with Plant Sterols Subjected to Adult and Elderly Digestion Conditions
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-07-22 , DOI: 10.1021/acs.jafc.4c03104
Virginia Blanco-Morales 1 , Dario Mercatante 2 , Nerea Faubel 1 , Diego Miedes 1 , Mara Mandrioli 2 , Maria Teresa Rodriguez-Estrada 2 , Guadalupe Garcia-Llatas 1
Affiliation  

This study evaluated the impact of different digestion conditions (adult and senior) on lipolysis and bioaccessibility of plant sterols (PS) and phytosterol oxidation products (POPs) in PS-enriched wholemeal rye bread. Under adult digestion conditions, the addition of gastric lipase (GL) reduced lipolysis products (by 6.1% for free fatty acids and 11.7% for monoacylglycerols) and the bioaccessibility of PS by 6.7%, compared to the control. In digestion with both GL and cholesterol esterase (CE), these reductions were 12.9, 20.1, and 11.3%, respectively. Both modifications (GL and GL + CE) increased the bioaccessibility of POPs by 4.5–4.0%. When simulating the elderly digestion, the modified gastric and intestinal phases did not alter PS bioaccessibility but decreased POPs bioaccessibility by 21.8% compared to control, along with reduced lipolysis. Incorporating GL and CE thus approached physiological conditions and influenced lipid digestion. Elderly simulated digestion conditions resulted in a positive outcome by maintaining PS bioaccessibility while reducing potentially harmful POPs.

中文翻译:


富含植物甾醇的全麦黑麦面包在成人和老年人消化条件下的脂肪分解和甾醇稳定性和生物可及性



本研究评估了不同消化条件(成人和老年人)对富含 PS 的全麦黑麦面包中植物甾醇 (PS) 和植物甾醇氧化产物 (POP) 的脂肪分解和生物可及性的影响。在成人消化条件下,与对照相比,添加胃脂肪酶(GL)可减少脂解产物(游离脂肪酸减少 6.1%,单酰甘油减少 11.7%),PS 的生物可利用性减少 6.7%。在用 GL 和胆固醇酯酶 (CE) 消化时,这些降低分别为 12.9%、20.1% 和 11.3%。两种修饰(GL 和 GL + CE)均将 POP 的生物可及性提高了 4.5-4.0%。当模拟老年人消化时,改良的胃和肠相并没有改变 PS 的生物可利用性,但与对照相比,POPs 的生物可利用性降低了 21.8%,同时脂肪分解也减少了。因此,加入 GL 和 CE 接近生理条件并影响脂质消化。老年人模拟消化条件通过保持 PS 生物可及性同时减少潜在有害的 POP 产生了积极的结果。
更新日期:2024-07-22
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