Itaconate is a promising platform chemical with broad applicability, including the synthesis of poly(methyl methacrylate). Most studies on microbial itaconate production entail the use of crop-based feedstock, which imposes constraints due to its limited supply. Brown macroalgae have recently gained attention as next-generation biomass owing to their high biomass productivity and carbohydrate content and amenability to mass production. Therefore, the use of macroalgae for itaconate production warrants exploration. In this study, the direct production of itaconate from brown macroalgae was demonstrated using engineered Vibrio sp. dhg, which has emerged as an efficient platform host for brown macroalgal biorefineries. Specifically, to enhance production, cis-aconitate decarboxylase (Cad) from Aspergillus terreus was heterologously expressed and isocitrate dehydrogenase (icd) was deleted. Notably, the resulting strain, VIC, achieved itaconate titers of 2.5 and 1.5 g/L from a mixture of alginate and mannitol (10 g/L of each) and 40 g/L of raw Saccharina japonica (S. japonica), respectively. Overall, this study highlights the utility of brown macroalgae as feedstock, as well as that of Vibrio sp. dhg as a platform strain for improving itaconate bioproduction.

中文翻译：

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使用工程弧菌直接从棕色大型藻类生产衣康酸。 DHG


衣康酸酯是一种有前景的平台化学品，具有广泛的适用性，包括聚甲基丙烯酸甲酯的合成。大多数关于微生物衣康酸生产的研究都需要使用农作物原料，但由于供应有限，这造成了限制。褐藻最近作为下一代生物质受到关注，因为它们具有高生物质生产率和碳水化合物含量以及适合大规模生产。因此，利用大型藻类生产衣康酸值得探索。在这项研究中，利用工程弧菌证明了从棕色大型藻类中直接生产衣康酸。 dhg，它已成为棕色大型藻生物精炼厂的有效平台宿主。具体来说，为了提高产量，异源表达来自土曲霉的顺乌头酸脱羧酶（Cad）并删除异柠檬酸脱氢酶（icd）。值得注意的是，所得菌株 VIC 从藻酸盐和甘露醇（各 10 g/L）的混合物以及 40 g/L 的原始日本糖（S. japonica）中分别获得了 2.5 和 1.5 g/L 的衣康酸滴度。总体而言，这项研究强调了棕色大型藻类以及弧菌作为原料的效用。 dhg 作为改善衣康酸生物生产的平台菌株。