Previous clinical studies indicate that monoamine oxidase-B (MAO-B) inhibition by blackcurrants must be predominantly attributed to bioactives other than anthocyanins. In this natural products discovery study, MAO-A/B inhibitory phytochemicals were isolated from blackcurrants, and a double-blind crossover study investigated the efficacy of freeze-dried whole-fruit blackcurrant powder in inhibiting MAO-B compared with blackcurrant juice in healthy adults. Platelet MAO-B inhibition was comparable between powder (89% ± 6) and juice (91% ± 4), and it was positively correlated with MAO-modulated plasma catecholamines, subjective alertness, and reduced mental fatigue, assessed using the Bond-Lader questionnaire. Sarmentosin, a nitrile glycoside, and its hydroxycinnamoyl esters were identified as novel MAO-A/B inhibitors from blackcurrant in vitro, and sarmentosin was demonstrated to inhibit platelet MAO-B activity in vivo. These findings confirm sarmentosin as the primary bioactive for MAO-A/B inhibition in blackcurrants, as well as its bioavailability and stability during freeze-drying, and suggest that consuming blackcurrant powder and juice may positively affect mood in healthy adults.

中文翻译：

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鉴定桑门妥辛是黑醋栗（Ribes nigrum）中抑制​​人类血小板单胺氧化酶的关键生物活性物质


先前的临床研究表明，黑加仑对单胺氧化酶 B (MAO-B) 的抑制作用主要归因于花青素以外的生物活性物质。在这项天然产物发现研究中，从黑加仑子中分离出了 MAO-A/B 抑制性植物化学物质，并进行了一项双盲交叉研究，研究了冻干全果黑加仑子粉与黑加仑子汁相比在健康成人中抑制 MAO-B 的功效。粉末 (89% ± 6) 和果汁 (91% ± 4) 之间的血小板 MAO-B 抑制作用相当，并且与 MAO 调节的血浆儿茶酚胺、主观警觉性和减少精神疲劳呈正相关（使用 Bond-Lader 进行评估）调查问卷。 Sarmentosin（一种腈糖苷）及其羟基肉桂酯在体外被鉴定为来自黑加仑的新型 MAO-A/B 抑制剂，并且在体内，Sarmentosin 被证明可以抑制血小板 MAO-B 活性。这些研究结果证实了垂柳菌素是黑加仑子中 MAO-A/B 抑制的主要生物活性物质，及其在冷冻干燥过程中的生物利用度和稳定性，并表明食用黑加仑子粉和果汁可能会对健康成年人的情绪产生积极影响。