ZbMYB111 Expression Positively Regulates ZbUFGT-Mediated Anthocyanin Biosynthesis in Zanthoxylum bungeanum with the Involvement of ZbbHLH2,Journal of Agricultural and Food Chemistry

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ZbMYB111 Expression Positively Regulates ZbUFGT-Mediated Anthocyanin Biosynthesis in Zanthoxylum bungeanum with the Involvement of ZbbHLH2
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-07-18 , DOI: 10.1021/acs.jafc.3c08579
Jie Zhang _{1,

2} , Nuan Han _{1,

2} , Aiguo Zhao _{1,

2} , Ziyi Wang _{1,

2} , Dongmei Wang _{1,

2}

Affiliation

Anthocyanin (ACN)-derived pigmentation in the red Zanthoxylum bungeanum peel is an essential commercial trait. Therefore, exploring the metabolic regulatory networks involved in peel ACN levels in this species is crucial for improving its quality. However, its underlying transcriptional regulatory mechanisms are still unknown. This transcriptomic and bioinformatics study not only discovered a new TF (ZbMYB111) as a potential regulator for ACN biosynthesis in Z. bungeanum peel, but also deciphered the underlying molecular mechanisms of ACN biosynthesis. Overexpression of ZbMYB111 and flavonoid 3-O-glucosyltransferase (ZbUFGT) induced ACN accumulation in both Z. bungeanum peels and callus along with Arabidopsis thaliana and tobacco flowers, whereas their silencing impaired ACN biosynthesis. Therefore, the dual-luciferase reporter, yeast-one-hybrid, and electrophoretic mobility shift assays showed that ZbMYB111 directly interacted with the ZbUFGT promoter to activate its expression. This diverted the secondary metabolism toward the ACN pathway, thereby promoting ACN accumulation.

中文翻译：

ZbMYB111 表达在 ZbbHLH2 的参与下正向调控 ZbUFGT 介导的花椒花青素生物合成

红花椒果皮中花青素 (ACN) 产生的色素沉着是一个重要的商业特征。因此，探索该物种果皮乙腈水平的代谢调控网络对于提高其质量至关重要。然而，其潜在的转录调控机制仍不清楚。这项转录组学和生物信息学研究不仅发现了一种新的转录因子（ ZbMYB111 ）作为花椒皮中乙腈生物合成的潜在调节因子，而且还破译了乙腈生物合成的潜在分子机制。 ZbMYB111和类黄酮 3-O-葡萄糖基转移酶( ZbUFGT ) 的过度表达诱导 ACN 在白皮苋皮和愈伤组织以及拟南芥和烟草花中积累，而它们的沉默会损害 ACN 生物合成。因此，双荧光素酶报告基因、酵母单杂交和电泳迁移率变动分析表明， ZbMYB111直接与ZbUFGT启动子相互作用以激活其表达。这将次级代谢转向乙腈途径，从而促进乙腈积累。

更新日期：2024-07-18

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