Dual Guardians of Immunity: FoRab10 and FoRab29 in Frankliniella occidentalis Confer Resistance to Tomato Spotted Wilt Orthotospovirus,Journal of Agricultural and Food Chemistry

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Dual Guardians of Immunity: FoRab10 and FoRab29 in Frankliniella occidentalis Confer Resistance to Tomato Spotted Wilt Orthotospovirus
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-07-18 , DOI: 10.1021/acs.jafc.4c03412
Xiaobin Zheng _{1,

2} , Jiangjiang Yuan ₁ , Yanran Wan ₃ , Yingxi Tang ₁ , Hongyi Cao ₁ , Jing Wang ₁ , Kanghua Qian ₁ , Ying Zhang ₁ , Sirui Chen ₁ , Baoyun Xu ₁ , Youjun Zhang ₁ , Pei Liang ₂ , Qingjun Wu ₁

Affiliation

Rab GTPase is critical for autophagy processes and is implicated in insect immunity against viruses. In this study, we aimed to investigate the role of FoRabs in the autophagic regulation of antiviral defense against tomato spotted wilt orthotospovirus (TSWV) in Frankliniella occidentalis. Transcriptome analysis revealed the downregulation of FoRabs in viruliferous nymph and adults of F. occidentalis in response to TSWV infection. Manipulation of autophagy levels with 3-MA and Rapa treatments resulted in a 5- to 15-fold increase and a 38–64% decrease in viral titers, respectively. Additionally, interference with FoRab10 in nymphs and FoRab29 in adults led to a 20–90% downregulation of autophagy-related genes, a decrease in ATG8-II (an autophagy marker protein), and an increase in the TSWV titers by 1.5- to 2.5-fold and 1.3- to 2.0-fold, respectively. In addition, the leaf disk and the living plant methods revealed increased transmission rates of 20.8–41.6 and 68.3–88.3%, respectively. In conclusion, FoRab10 and FoRab29 play a role in the autophagic regulation of the antiviral defense in F. occidentalis nymphs and adults against TSWV, respectively. These findings offer insights into the intricate immune mechanisms functional in F. occidentalis against TSWV, suggesting potential targeted strategies for F. occidentalis and TSWV management.

中文翻译：

免疫的双重守护者：西花蓟马中的 FoRab10 和 FoRab29 赋予对番茄斑萎正孢病毒的抗性

Rab GTP 酶对于自噬过程至关重要，并且与昆虫对病毒的免疫有关。在本研究中，我们旨在研究FoRabs在西花蓟马 ( Frankliniella occidentalis)中针对番茄斑萎正孢病毒 (TSWV) 的抗病毒防御自噬调节中的作用。转录组分析显示，西方 F. occidentalis的带病毒若虫和成虫中， FoRabs因 TSWV 感染而下调。通过 3-MA 和 Rapa 处理控制自噬水平，病毒滴度分别增加 5 至 15 倍和下降 38-64%。此外，干扰若虫中的FoRab10和成虫中的FoRab29会导致自噬相关基因下调 20-90%，ATG8-II（自噬标记蛋白）减少，TSWV 滴度增加 1.5-2.5分别为-倍和1.3-2.0倍。此外，叶盘和活植物方法显示传播率分别增加了 20.8-41.6 和 68.3-88.3%。总之，FoRab10 和 FoRab29 分别在F. occidentalis若虫和成虫针对 TSWV 的抗病毒防御的自噬调节中发挥作用。这些发现提供了对F. occidentalis对抗 TSWV 的复杂免疫机制的见解，提出了F. occidentalis和 TSWV 管理的潜在针对性策略。

更新日期：2024-07-18

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