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Exploration of GmDof11-lncRNA13082 Module Regulating Oil Synthesis in Plants
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-07-17 , DOI: 10.1021/acs.jafc.4c03084 Qiuzhu Zhao 1, 2 , Dingyi Zhao 2 , Yashuo Wang 1 , Yuxin Li 1 , Chang Ni 1 , Zitong Su 1 , Panhang Lian 1 , Shuying Liu 1 , Huijing Liu 1 , Jun Zhang 2 , Dan Yao 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-07-17 , DOI: 10.1021/acs.jafc.4c03084 Qiuzhu Zhao 1, 2 , Dingyi Zhao 2 , Yashuo Wang 1 , Yuxin Li 1 , Chang Ni 1 , Zitong Su 1 , Panhang Lian 1 , Shuying Liu 1 , Huijing Liu 1 , Jun Zhang 2 , Dan Yao 1
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Soybean (Glycine max [Linn.] Merr.) is an important oilseed crop. Although transcription factors (TFs) can coordinate the expression of mRNA and lncRNA, their coordination in the soybean oil synthesis pathway remains unclear. This study examined the interaction between the TF GmDof11 and lncRNA13082 and found that overexpression of GmDof11 led to an increase in the number of Arabidopsis seeds, thousand seed weight, crude protein, hydrolysis amino acid, and soluble sugar. Additionally, it reduced the triglyceride and starch contents and affected the proportion of fatty acids, increasing the contents of palmitic acid, stearic acid, and linolenic acid. The yeast two-hybrid experiments revealed that GmDof11 interacts with GmBCCP1, GmLEC1b, and GmFAB2 proteins. In the RT-qPCR analysis of transgenic soybean roots, it was found that GmDof11 can activate the production of lncRNA13082 and work in conjunction with lncRNA13082 to oversee oil synthesis and nutrient storage. Our research provides robust theoretical evidence for a comprehensive resolution of TF-lncRNA regulation in the soybean oil synthesis network.
中文翻译：
GmDof11-lncRNA13082模块调控植物油合成的探索
大豆（ Glycine max [Linn.] Merr.）是一种重要的油料作物。尽管转录因子（TF）可以协调mRNA和lncRNA的表达，但它们在大豆油合成途径中的协调仍不清楚。本研究检测了TF GmDof11和lncRNA13082之间的相互作用，发现GmDof11的过度表达导致拟南芥种子数量、千粒重、粗蛋白、水解氨基酸和可溶性糖的增加。此外，它还降低了甘油三酯和淀粉含量，影响了脂肪酸的比例，增加了棕榈酸、硬脂酸和亚麻酸的含量。酵母双杂交实验表明，GmDof11 与 GmBCCP1、GmLEC1b 和 GmFAB2 蛋白相互作用。在转基因大豆根部的 RT-qPCR 分析中，发现 GmDof11 可以激活lncRNA13082的产生，并与lncRNA13082协同作用，监督油脂合成和养分储存。我们的研究为全面解决大豆油合成网络中 TF- lncRNA调控提供了强有力的理论证据。
更新日期：2024-07-17
中文翻译：
GmDof11-lncRNA13082模块调控植物油合成的探索
大豆（ Glycine max [Linn.] Merr.）是一种重要的油料作物。尽管转录因子（TF）可以协调mRNA和lncRNA的表达，但它们在大豆油合成途径中的协调仍不清楚。本研究检测了TF GmDof11和lncRNA13082之间的相互作用，发现GmDof11的过度表达导致拟南芥种子数量、千粒重、粗蛋白、水解氨基酸和可溶性糖的增加。此外，它还降低了甘油三酯和淀粉含量，影响了脂肪酸的比例，增加了棕榈酸、硬脂酸和亚麻酸的含量。酵母双杂交实验表明，GmDof11 与 GmBCCP1、GmLEC1b 和 GmFAB2 蛋白相互作用。在转基因大豆根部的 RT-qPCR 分析中，发现 GmDof11 可以激活lncRNA13082的产生，并与lncRNA13082协同作用，监督油脂合成和养分储存。我们的研究为全面解决大豆油合成网络中 TF- lncRNA调控提供了强有力的理论证据。
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