Dissecting the role of the MS-ring protein FliF in Bacillus cereus flagella-related functions,Molecular Microbiology

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Dissecting the role of the MS-ring protein FliF in Bacillus cereus flagella-related functions
Molecular Microbiology ( IF 2.6 ) Pub Date : 2024-07-19 , DOI: 10.1111/mmi.15299
Diletta Mazzantini ₁ , Guendalina Gherardini ₁ , Virginia Rossi ₁ , Francesco Celandroni ₁ , Marco Calvigioni ₁ , Adelaide Panattoni ₁ , Mariacristina Massimino ₁ , Antonella Lupetti ₁ , Emilia Ghelardi _{1,

2}

Affiliation

The flagellar MS-ring, uniquely constituted by FliF, is essential for flagellar biogenesis and functionality in several bacteria. The aim of this study was to dissect the role of FliF in the Gram-positive and peritrichously flagellated Bacillus cereus. We demonstrate that fliF forms an operon with the upstream gene fliE. In silico analysis of B. cereus ATCC 14579 FliF identifies functional domains and amino acid residues that are essential for protein functioning. The analysis of a ΔfliF mutant of B. cereus, constructed in this study using an in frame markerless gene replacement method, reveals that the mutant is unexpectedly able to assemble flagella, although in reduced amounts compared to the parental strain. Nevertheless, motility is completely abolished by fliF deletion. FliF deprivation causes the production of submerged biofilms and affects the ability of B. cereus to adhere to gastrointestinal mucins. We additionally show that the fliF deletion does not compromise the secretion of the three components of hemolysin BL, a toxin secreted through the flagellar type III secretion system. Overall, our findings highlight the important role of B. cereus FliF in flagella-related functions, being the protein required for complete flagellation, motility, mucin adhesion, and pellicle biofilms.

中文翻译：

剖析 MS 环蛋白 FliF 在蜡样芽胞杆菌鞭毛相关功能中的作用

鞭毛 MS 环由 FliF 独特构成，对于多种细菌的鞭毛生物发生和功能至关重要。本研究的目的是剖析 FliF 在革兰氏阳性且周毛有鞭毛的蜡样芽孢杆菌中的作用。我们证明fliF与上游基因fliE形成操纵子。对蜡样芽胞杆菌ATCC 14579 FliF 进行计算机分析，识别蛋白质功能所必需的功能域和氨基酸残基。本研究中使用框内无标记基因替换方法构建的蜡样芽孢杆菌Δ fliF突变体的分析表明，该突变体出乎意料地能够组装鞭毛，尽管与亲本菌株相比，组装量有所减少。然而， fliF缺失完全消除了运动性。 FliF 剥夺会导致淹没生物膜的产生，并影响蜡样芽孢杆菌粘附胃肠道粘蛋白的能力。我们还表明， fliF缺失不会影响溶血素 BL 三种成分的分泌，溶血素 BL 是一种通过鞭毛 III 型分泌系统分泌的毒素。总的来说，我们的研究结果强调了蜡样芽孢杆菌FliF 在鞭毛相关功能中的重要作用，它是完整鞭毛、运动、粘蛋白粘附和表膜生物膜所需的蛋白质。

更新日期：2024-07-19

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