Novel mycophenolic acid precursor-based fluorescent probe for intracellular H2O2 detection in living cells and Daphnia magna and Zebrafish model systems,Analyst

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Novel mycophenolic acid precursor-based fluorescent probe for intracellular H2O2 detection in living cells and Daphnia magna and Zebrafish model systems
Analyst ( IF 3.6 ) Pub Date : 2024-07-18 , DOI: 10.1039/d4an00742e
Jongkeol An ₁ , Sujeong Park ₂ , Neha Jain ₁ , Youngsam Kim _{3,

4} , Satish Balasaheb Nimse ₂ , David G Churchill _{1,

5}

Affiliation

Innovative for the scientific community and attracting attention in the extensive biomedical field are novel compact organic chemosensing systems built upon unique core molecular frameworks. These systems may demonstrate customized responses and may be adaptable to analytes, showing promise for potential in vivo applications. Our recent investigation focuses on a precursor of Mycophenolic acid, resulting in the development of LBM (LOD = 13 nM) – a specialized probe selective for H₂O₂. This paper details the synthesis, characterization, and thorough biological assessments of LBM. Notably, we conducted experiments involving living cells, daphnia, and zebrafish models, utilizing microscopy techniques to determine probe nontoxicity and discern distinct patterns of probe localization. Localization involved the distribution of the probe in the Zebrafish model within the gut, esophagus, and muscles of the antennae.

中文翻译：

用于活细胞、大型水蚤和斑马鱼模型系统中细胞内 H2O2 检测的新型霉酚酸前体荧光探针

基于独特核心分子框架的新型紧凑有机化学传感系统对科学界具有创新意义，并在广泛的生物医学领域引起了人们的关注。这些系统可以表现出定制的响应，并且可以适应分析物，显示出潜在的体内应用的前景。我们最近的研究重点是霉酚酸的前体，从而开发了LBM (LOD = 13 nM)——一种选择性 H ₂ O ₂的专用探针。本文详细介绍了LBM的合成、表征和彻底的生物学评估。值得注意的是，我们进行了涉及活细胞、水蚤和斑马鱼模型的实验，利用显微镜技术来确定探针的无毒性并辨别探针定位的不同模式。定位涉及斑马鱼模型中探针在肠道、食道和触角肌肉内的分布。

更新日期：2024-07-23

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