As the global community seeks cleaner and sustainable energy alternatives, this study explores the potential of garbage enzymes (GE) derived from fruit peels, enriched with sheep feces amendment, for bio-hydrogen production from waste-activated sludge (WAS). The optimized GE fermented at 52 °C for 6 days with 3.8% wt/v sheep feces, exhibited maximum biocatalytic activity, including protease, amylase, and lipase activities, resulting in enhanced WAS hydrolysis. Application of the optimized GE at 52 °C for 5 days led to significant suspended solid (SS) removal (60.97%) and soluble chemical oxygen demand (sCOD) solubilization (165.45%) in WAS. Additionally, bio-hydrogen production peaked at a 30% v/v dosage concentration of optimized GE on WAS. These findings highlight the potential of GE to transform WAS into a more bioavailable form for efficient and sustainable bio-hydrogen production. The study contributes to the development of cost-effective enzymes derived from organic wastes, capable of hydrolyzing WAS into a more bioavailable form and facilitating bio-hydrogen production.

Graphical Abstract

中文翻译：

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探索果皮和羊粪便垃圾酶用于污水污泥制氢的潜力

随着全球社会寻求更清洁和可持续的能源替代品，这项研究探讨了从果皮中提取的垃圾酶（GE），富含羊粪便改良剂，用于从废物活性污泥（WAS）生产生物氢的潜力。优化后的GE在52℃下用3.8％wt/v的羊粪便发酵6天，表现出最大的生物催化活性，包括蛋白酶、淀粉酶和脂肪酶活性，从而增强WAS水解。在 52 °C 下应用优化的 GE 5 天，WAS 中的悬浮固体 (SS) 显着去除 (60.97%)，可溶性化学需氧量 (sCOD) 溶解 (165.45%)。此外，WAS 上优化 GE 的剂量浓度为 30% v/v 时，生物氢产量达到峰值。这些发现凸显了 GE 将 WAS 转化为生物利用度更高的形式以实现高效、可持续的生物制氢的潜力。该研究有助于开发从有机废物中提取的具有成本效益的酶，能够将 WAS 水解成生物可利用性更高的形式，并促进生物氢的生产。

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