Fluorescent probe for imaging intercellular tension: molecular force approach,RSC Advances

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Fluorescent probe for imaging intercellular tension: molecular force approach
RSC Advances ( IF 3.9 ) Pub Date : 2024-07-19 , DOI: 10.1039/d4ra02647k
Xiao-Hong Wang ₁ , Ming Wang ₂ , Jian-Bin Pan ₃ , Jin-Miao Zhu ₁ , Hu Cheng ₁ , Hua-Ze Dong ₁ , Wen-Jie Bi ₁ , Shi-Wei Yang ₁ , Yuan-Yuan Chen ₁ , Fan Xu ₁ , Xiao-Jing Duan ₁

Affiliation

Cellular mechanical force plays a crucial role in numerous biological processes, including wound healing, cell development, and metastasis. To enable imaging of intercellular tension, molecular tension probes were designed, which offer a simple and efficient method for preparing Au-DNA intercellular tension probes with universal applicability. The proposed approach utilizes gold nanoparticles linked to DNA hairpins, enabling sensitive visualization of cellular force in vitro. Specifically, the designed Au-DNA intercellular tension probe includes a molecular spring flanked by a fluorophore–quencher pair, which is anchored between cells. As intercellular forces open the hairpin, the fluorophore is de-quenched, allowing for visualization of cellular force. The effectiveness of this approach was demonstrated by imaging the cellular force in living cells using the designed Au-DNA intercellular tension probe.

中文翻译：

用于细胞间张力成像的荧光探针：分子力方法

细胞机械力在许多生物过程中发挥着至关重要的作用，包括伤口愈合、细胞发育和转移。为了实现细胞间张力成像，设计了分子张力探针，为制备具有普遍适用性的Au-DNA细胞间张力探针提供了一种简单有效的方法。所提出的方法利用与 DNA 发夹连接的金纳米颗粒，能够在体外对细胞力进行灵敏的可视化。具体来说，设计的 Au-DNA 细胞间张力探针包括一个分子弹簧，其两侧是荧光团-猝灭剂对，固定在细胞之间。当细胞间力打开发夹时，荧光团被去猝灭，从而使细胞力可视化。通过使用设计的 Au-DNA 细胞间张力探针对活细胞中的细胞力进行成像，证明了该方法的有效性。

更新日期：2024-07-19

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