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Toxicity and related molecular mechanisms of Sb(III) in the embryos and larvae of zebrafish (Danio rerio)
Environmental Pollution ( IF 7.6 ) Pub Date : 2024-07-14 , DOI: 10.1016/j.envpol.2024.124551 Ziyang Lai 1 , Yihan Wei 1 , Mengchang He 1 , Chunye Lin 1 , Wei Ouyang 2 , Xitao Liu 1
Environmental Pollution ( IF 7.6 ) Pub Date : 2024-07-14 , DOI: 10.1016/j.envpol.2024.124551 Ziyang Lai 1 , Yihan Wei 1 , Mengchang He 1 , Chunye Lin 1 , Wei Ouyang 2 , Xitao Liu 1
Affiliation
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Antimony (Sb) pollution poses a severe threat to humans and ecosystems due to the extensive use of Sb in various fields. However, little is known about the toxic effects of Sb and its aquatic ecotoxicological mechanism. This study aimed to reveal the toxicity and related molecular mechanisms of trivalent Sb (Sb(III)) in zebrafish embryos/larvae. Sb(III) accumulated in larvae, which correlated with the exposure concentration. Although no significant lethal or teratogenic effects were observed, normal growth and development were affected. Exposure to 10 or 20 mg/L Sb(III) increased the levels of reactive oxygen species in the larvae while enhancing catalase activity and increasing cell apoptosis. Transcriptomic analysis revealed that Sb(III) promoted glutathione metabolism and the ferroptosis pathway. In addition, symptoms associated with ferroptosis, including mitochondrial damage, biochemical levels of related molecules and increased tissue iron content, were detected. Quantitative polymerase chain reaction (qPCR) analyses further confirmed that Sb(III) significantly altered the transcription levels of genes related to the ferroptosis pathway by disrupting iron homeostasis. Furthermore, ferrostatin-1 (Fer-1) mitigated the toxic effects induced by Sb(III) in zebrafish. Our research fills the gap in the literature on the toxicity and mechanism of Sb(III) in aquatic organisms, which is highly important for understanding the ecological risks associated with Sb.
中文翻译：
Sb(III)对斑马鱼胚胎和幼鱼的毒性及相关分子机制
由于锑在各个领域的广泛使用，锑（Sb）污染对人类和生态系统构成了严重威胁。然而，人们对锑的毒性作用及其水生生态毒理学机制知之甚少。本研究旨在揭示三价锑（Sb(III)）对斑马鱼胚胎/幼虫的毒性及相关分子机制。 Sb(III)在幼虫体内积累，与暴露浓度相关。虽然没有观察到明显的致死或致畸作用，但正常生长和发育受到影响。暴露于 10 或 20 mg/L Sb(III) 会增加幼虫中活性氧的水平，同时增强过氧化氢酶活性并增加细胞凋亡。转录组分析表明，Sb(III) 促进谷胱甘肽代谢和铁死亡途径。此外，还检测到与铁死亡相关的症状，包括线粒体损伤、相关分子的生化水平和组织铁含量增加。定量聚合酶链反应 (qPCR) 分析进一步证实，Sb(III) 通过破坏铁稳态，显着改变与铁死亡途径相关的基因的转录水平。此外，ferrostatin-1 (Fer-1) 减轻了 Sb(III) 对斑马鱼引起的毒性作用。我们的研究填补了有关Sb(III)对水生生物的毒性和机制的文献空白，这对于了解Sb相关的生态风险非常重要。
更新日期：2024-07-14
中文翻译：
Sb(III)对斑马鱼胚胎和幼鱼的毒性及相关分子机制
由于锑在各个领域的广泛使用，锑（Sb）污染对人类和生态系统构成了严重威胁。然而，人们对锑的毒性作用及其水生生态毒理学机制知之甚少。本研究旨在揭示三价锑（Sb(III)）对斑马鱼胚胎/幼虫的毒性及相关分子机制。 Sb(III)在幼虫体内积累，与暴露浓度相关。虽然没有观察到明显的致死或致畸作用，但正常生长和发育受到影响。暴露于 10 或 20 mg/L Sb(III) 会增加幼虫中活性氧的水平，同时增强过氧化氢酶活性并增加细胞凋亡。转录组分析表明，Sb(III) 促进谷胱甘肽代谢和铁死亡途径。此外，还检测到与铁死亡相关的症状，包括线粒体损伤、相关分子的生化水平和组织铁含量增加。定量聚合酶链反应 (qPCR) 分析进一步证实，Sb(III) 通过破坏铁稳态，显着改变与铁死亡途径相关的基因的转录水平。此外，ferrostatin-1 (Fer-1) 减轻了 Sb(III) 对斑马鱼引起的毒性作用。我们的研究填补了有关Sb(III)对水生生物的毒性和机制的文献空白，这对于了解Sb相关的生态风险非常重要。
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