BackgroundChlorhexidine (CHX)‐based mouth rinses are frequently prescribed following periodontal surgeries. A more recently available brand of zinc‐based mouth rinses advertises one of its mouth rinses as a substitute for chlorhexidine. The purpose of this study was to evaluate, in vitro, the effects of this brand of zinc‐based mouth rinses on cell survival, cell motility, and gene expression of human gingival fibroblasts (HGFs).MethodsHGFs were exposed to essential oil (EO), CHX, and three types of one brand of zinc‐based mouth rinses designed to treat breath malodor (ZnA), dry mouth (ZnB), and gingivitis (ZnC). Each mouth rinse was tested over a range of concentrations for its effects on HGF survival and motility. Gene expression of cytokines, interleukins, and growth factors were evaluated via reverse transcriptase–polymerase chain reaction (RT‐PCR), as a means to assess potential influences on inflammation and wound healing.ResultsCell survival was significantly decreased for CHX and ZnC at 10% dilutions (p < 0.05). For all time points, cells exposed to ZnC displayed the greatest reduction in cell motility (p < 0.05). The various mouth rinses examined differentially altered the expression of growth factor transcripts. ZnC particularly enhanced the expression of BMP‐2 and FGF‐2.ConclusionZnC was more cytotoxic and inhibited cell motility to a greater extent than any of the other mouth rinses. Therefore, using ZnC as an alternative to CHX could potentially have negative effects on wound healing after periodontal surgery. However, further investigation is required to confirm the clinical relevance of these in vitro findings.PLAIN LANGUAGE SUMMARYOne type of zinc‐based mouth rinse designed to replace chlorhexidine (often prescribed after oral surgeries) demonstrated the greatest oral cell death and reduction in cell movement when compared to other zinc‐based mouth rinses. These zinc‐based mouth rinses also reduced the amounts of proteins involved in regulating inflammation, potentially reducing the destruction of bone holding the teeth in place. They also changed the amounts of several molecules involved in tissue healing. It is unknown if this will speed or slow the healing of the soft tissues of the mouth.

中文翻译：

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锌基漱口水、氯己定漱口水和精油漱口水的体外比较


背景 牙周手术后经常使用基于洗必泰 (CHX) 的漱口水。最近推出的一种锌基漱口水品牌在广告中宣传其一种漱口水作为洗必泰的替代品。本研究的目的是在体外评估该品牌的锌基漱口水对人牙龈成纤维细胞 (HGF) 的细胞存活、细胞运动和基因表达的影响。方法将 HGF 暴露于精油 (EO) 、CHX 和同一个品牌的三种锌基漱口水，旨在治疗口臭 (ZnA)、口干 (ZnB) 和牙龈炎 (ZnC)。每种漱口水均在一定浓度范围内测试其对 HGF 存活和活力的影响。通过逆转录酶聚合酶链式反应 (RT-PCR) 评估细胞因子、白细胞介素和生长因子的基因表达，作为评估对炎症和伤口愈合的潜在影响的方法。结果 CHX 和 ZnC 在 10% 时细胞存活率显着下降稀释度（ p < 0.05）。对于所有时间点，暴露于 ZnC 的细胞显示出细胞运动性的最大降低（ p < 0.05）。所检查的各种漱口水不同程度地改变了生长因子转录物的表达。 ZnC 特别增强了 BMP-2 和 FGF-2 的表达。结论 ZnC 比任何其他漱口水具有更强的细胞毒性和更大程度地抑制细胞运动。因此，使用 ZnC 作为 CHX 的替代品可能会对牙周手术后伤口愈合产生负面影响。然而，需要进一步研究来确认这些体外研究结果的临床相关性。通俗语言摘要 与其他锌基漱口水相比，一种旨在替代氯己定（通常在口腔手术后开处方）的锌基漱口水显示出最大的口腔细胞死亡和细胞运动减少。这些含锌漱口水还减少了参与调节炎症的蛋白质含量，可能减少固定牙齿的骨骼的破坏。他们还改变了参与组织愈合的几种分子的数量。目前尚不清楚这是否会加速或减缓口腔软组织的愈合。