Hypochlorite (ClO⁻) is an important oxidant and disinfectant in the human body and in nature. Nevertheless, it may exert an adverse impact on the environment and biological systems when its concentration is not appropriate. It is necessary to develop an accurate method for detecting ClO⁻. Herein, an isopinocamphone-based fluorescent probe DMTI for selective recognition of ClO⁻ was successfully synthesized. DMTI exhibited an obvious fluorescence enhancement towards ClO⁻ accompanied by a color shift from colorless to bright blue. The experimental results displayed that the probe DMTI had the merits of high selectivity, good sensitivity, and a wide detection pH range (6–10) for ClO⁻ detection, and the detection limit reached 2.6 × 10⁻⁷ mol L⁻¹. The sensing mechanism of DMTI to ClO⁻ was confirmed by the ¹H NMR and HRMS analyses. More importantly, the probe DMTI has been effectively utilized to detect ClO⁻ in environmental water samples, living cells and zebrafish encouraged by its excellent performance.

中文翻译：

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一种新型异蒎蒎酮荧光探针的构建及其在环境分析和生物成像中的应用


次氯酸盐（ClO ⁻ ）是人体和自然界中重要的氧化剂和消毒剂。然而，当其浓度不合适时，可能会对环境和生物系统产生不利影响。有必要开发准确的ClO ⁻ 检测方法。在此，成功合成了基于异蒎莰酮的选择性识别ClO ⁻ 的荧光探针DMTI。 DMTI 对 ClO ⁻ 表现出明显的荧光增强，并伴有从无色到亮蓝色的色移。实验结果表明，探针DMTI对ClO ⁻ 的检测具有选择性高、灵敏度好、检测pH范围宽（6~10）等优点，检出限达到2.6×10 ⁻⁷ mol L ⁻¹ 。 ¹ H NMR 和 HRMS 分析证实了 DMTI 对 ClO ⁻ 的传感机制。更重要的是，探针DMTI因其优异的性能已被有效地用于检测环境水样、活细胞和斑马鱼中的ClO ⁻ 。