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Modulating Visible-Light Driven NIR Lanthanide Polymer Photocatalysis for Amplification Detection of Exosomal Proteins and Cancer Diagnosis
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-07-13 , DOI: 10.1021/acs.analchem.4c02168 Haiyan Li 1 , Yafei Chen 1 , Qing Gao 1 , Nan Wang 2 , Ting Yang 1 , Cheng Du 3 , Mingli Chen 1, 2 , Jianhua Wang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-07-13 , DOI: 10.1021/acs.analchem.4c02168 Haiyan Li 1 , Yafei Chen 1 , Qing Gao 1 , Nan Wang 2 , Ting Yang 1 , Cheng Du 3 , Mingli Chen 1, 2 , Jianhua Wang 1
Affiliation
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Near-infrared (NIR) luminescent lanthanide materials hold great promise for bioanalysis, as they have anti-interference properties. The approach of efficient luminescence is sensitization through a reasonable chromophore to overcome the obstacle of the aqueous phase. The involvement of the surfactant motif is an innovative strategy to arrange the amphiphilic groups to be regularly distributed near the polymer to form a closed sensitized space. Herein, a lanthanide polymer (TCPP-PEI70K-FITC@Yb/SDBS) is designed in which the meso-tetra(4-carboxyphenyl)porphine (TCPP) ligand serves as both a sensitizer and photocatalytic switch. The surfactant sodium dodecyl benzenesulfonate (SDBS) wraps the photosensitive polymers to form a hydrophobic layer, which augments the light-harvesting ability and expedites its photocatalysis. TCPP-PEI70K-FITC@Yb/SDBS is subsequently applied as an amplified photocatalysis toolbox for universally regulating the generation of reactive oxygen species (ROS). Boosting 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation to produce blue products, a dual-mode biosensor is fabricated for improving the diagnosis of programmed death ligand-1-positive (PDL1) cancer exosomes. Exosomes were captured by Fe3O4 modified by the PDL1 aptamer, enabling replacement of alkaline phosphatase (ALP)-labeled multiple hybridized chains; then, the isolated ALP triggered a hydrolysis reaction to block the generation of oxTMB. Detection sensitivity improves by 1 order of magnitude through SDBS modulation, down to 104 particles/mL. The sensor performed well clinically in distinguishing cancer patients from healthy individuals, expanding physiological applications of near-infrared lanthanide luminescence.
中文翻译：
调节可见光驱动的近红外镧系聚合物光催化用于外泌体蛋白的扩增检测和癌症诊断
近红外（NIR）发光稀土材料具有抗干扰特性，因此在生物分析方面具有广阔的前景。高效发光的途径是通过合理的发色团进行敏化，克服水相的障碍。表面活性剂基序的参与是一种创新策略，将两亲基团规则地分布在聚合物附近，形成封闭的敏化空间。在此，设计了一种镧系聚合物（TCPP-PEI 70K -FITC@Yb/SDBS），其中内消旋四（4-羧基苯基）卟啉（TCPP）配体既充当敏化剂又充当光催化开关。表面活性剂十二烷基苯磺酸钠（SDBS）包裹光敏聚合物形成疏水层，增强光捕获能力并加速其光催化作用。 TCPP-PEI 70K -FITC@Yb/SDBS 随后被用作放大光催化工具箱，用于普遍调节活性氧 (ROS) 的产生。一种双模式生物传感器促进 3,3',5,5'-四甲基联苯胺 (TMB) 氧化产生蓝色产物，用于改善程序性死亡配体 1 阳性 (PDL1) 癌症外泌体的诊断。外泌体被 PDL1 适体修饰的 Fe 3 O 4捕获，能够替换碱性磷酸酶（ALP）标记的多条杂交链；然后，分离出的 ALP 引发水解反应，阻止 oxTMB 的生成。通过 SDBS 调制，检测灵敏度提高了 1 个数量级，降至 10 4颗粒/mL。 该传感器在临床上区分癌症患者和健康个体方面表现良好，扩展了近红外镧系元素发光的生理应用。
更新日期：2024-07-13
中文翻译：
调节可见光驱动的近红外镧系聚合物光催化用于外泌体蛋白的扩增检测和癌症诊断
近红外（NIR）发光稀土材料具有抗干扰特性，因此在生物分析方面具有广阔的前景。高效发光的途径是通过合理的发色团进行敏化，克服水相的障碍。表面活性剂基序的参与是一种创新策略，将两亲基团规则地分布在聚合物附近，形成封闭的敏化空间。在此，设计了一种镧系聚合物（TCPP-PEI 70K -FITC@Yb/SDBS），其中内消旋四（4-羧基苯基）卟啉（TCPP）配体既充当敏化剂又充当光催化开关。表面活性剂十二烷基苯磺酸钠（SDBS）包裹光敏聚合物形成疏水层，增强光捕获能力并加速其光催化作用。 TCPP-PEI 70K -FITC@Yb/SDBS 随后被用作放大光催化工具箱，用于普遍调节活性氧 (ROS) 的产生。一种双模式生物传感器促进 3,3',5,5'-四甲基联苯胺 (TMB) 氧化产生蓝色产物，用于改善程序性死亡配体 1 阳性 (PDL1) 癌症外泌体的诊断。外泌体被 PDL1 适体修饰的 Fe 3 O 4捕获，能够替换碱性磷酸酶（ALP）标记的多条杂交链；然后，分离出的 ALP 引发水解反应，阻止 oxTMB 的生成。通过 SDBS 调制，检测灵敏度提高了 1 个数量级，降至 10 4颗粒/mL。 该传感器在临床上区分癌症患者和健康个体方面表现良好，扩展了近红外镧系元素发光的生理应用。
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