Cadmium (Cd) accumulates in rice and then moves up the food chain, causing serious health problems for humans. Glutathione S-transferase (GST) binds exogenous hazardous compounds to glutathione (GSH), which performs a variety of roles in plant responses to Cd stress. Here, Cd stimulated the transcripts of a novel OsGST gene, and the OsGST protein, which was localized in the nucleus and cytoplasm, was also induced by Cd. In OsGST deletion mutant lines generated by CRISPR/Cas9, more Cd was accumulated, and Cd hypersensitive phenotypes were observed, while transgenic lines overexpressing OsGST exhibited enhanced Cd tolerance and less Cd accumulation. Further analysis indicated that the osgst mutants exhibited considerably greater reactive oxygen species (ROS) and higher GSH level, and the antioxidant activity associated genes’ expression were down-regulated, imply that OsGST controlled rice Cd accumulation and resistance through preserving the equilibrium of the GSH and redox in rice.

中文翻译：

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编码 9 谷胱甘肽还原酶的新型 OsGST 基因负向调节水稻镉积累


镉（Cd）在大米中积累，然后沿着食物链向上移动，给人类带来严重的健康问题。谷胱甘肽 S-转移酶 (GST) 将外源有害化合物与谷胱甘肽 (GSH) 结合，谷胱甘肽在植物对镉胁迫的反应中发挥多种作用。在这里，Cd 刺激了一种新的 OsGST 基因的转录，并且位于细胞核和细胞质中的 OsGST 蛋白也被 Cd 诱导。在CRISPR/Cas9产生的OsGST缺失突变株系中，积累了更多的Cd，并观察到Cd过敏表型，而过表达OsGST的转基因株系表现出增强的Cd耐受性和更少的Cd积累。进一步分析表明，osgst突变体表现出更高的活性氧（ROS）和更高的GSH水平，并且抗氧化活性相关基因的表达下调，这表明OsGST通过保持GSH平衡来控制水稻Cd积累和抗性。以及大米中的氧化还原。