Saponin is one of the main active ingredients of Polygonatum sibiricum. In this study, the method of enzyme-assisted cold isostatic pressure extraction (EACIP) of Polygonatum sibiricum saponins was established. The effects of different cold isostatic extraction pressures, pressure-maintaining time times, enzymatic temperatures and enzymatic pH on the yield of saponin were investigated, and the factors were simplified by single factor experiments and Box-benhken design (BBD). Under the best extraction conditions, the EACIP method achieved the best possible results and extraction rate was 11.45 % ± 0.017. In addition, SEM-Mapping, XRD analysis and FTIR analysis proved that EACIP treatment increased the extent of cell deformation and improved the mass transfer system between solvent and active substance. Furthermore, by comparing different extraction methods, EACIP was found to have a higher extraction rate due to the synergistic effect of cold isostatic pressure extraction (CIP) and enzymatic hydrolysis extraction (EH). In vitro antioxidant and anti-inflammatory assays demonstrated that EACIP extracts have good antioxidant and anti-inflammatory capacity. Therefore, EACIP is a method to extract natural products from plants with increased extraction rate.

中文翻译：

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酶辅助冷等静压技术高效提取西伯利亚黄精皂苷的研究


皂苷是黄精的主要活性成分之一。本研究建立了酶辅助冷等静压提取黄精皂苷的方法。考察了不同冷等静压提取压力、保压时间、酶解温度和酶解pH对皂苷得率的影响，并通过单因素实验和Box-benhken设计（BBD）简化了因素。在最佳提取条件下，EACIP法取得了最好的结果，提取率为11.45%±0.017。此外，SEM-Mapping、XRD 分析和 FTIR 分析证明，EACIP 处理增加了细胞变形程度，改善了溶剂和活性物质之间的传质系统。此外，通过比较不同的提取方法，发现由于冷等静压提取（CIP）和酶水解提取（EH）的协同作用，EACIP具有更高的提取率。体外抗氧化和抗炎实验表明，EACIP提取物具有良好的抗氧化和抗炎能力。因此，EACIP是一种提高提取率的从植物中提取天然产物的方法。