The soil-borne bacterial pathogen Ralstonia solanacearum causes significant losses in Solanaceae crop production worldwide, including tomato, potato, and eggplant. To efficiently prevent outbreaks, it is essential to understand the complex interactions between pathogens and the microbiome. One promising mechanism for enhancing microbiome functionality is siderophore-mediated competition, which is shaped by the low iron availability in the rhizosphere. This study explores the critical role of iron competition in determining microbiome functionality and its potential for designing high-performance microbiome engineering strategies. We investigated the impact of siderophore-mediated interactions on the efficacy of Pseudomonas spp. consortia in suppressing R. solanacearum, both in vitro and in vivo. Our findings show that siderophore production significantly enhances the inhibitory effects of Pseudomonas strains on pathogen growth, while other metabolites are less effective under iron-limited conditions. Moreover, siderophores play a crucial role in shaping interactions within the consortia, ultimately determining the level of protection against bacterial wilt disease. This study highlights the key role of siderophores in mediating consortium interactions and their impact on tomato health. Our results also emphasize the limited efficacy of other secondary metabolites in iron-limited environments, underscoring the importance of siderophore-mediated competition in maintaining tomato health and suppressing disease.

中文翻译：

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铁载体相互作用驱动假单胞菌的能力。保护番茄免受青枯菌侵害的联盟


土传细菌病原体青枯雷尔斯顿氏菌（Ralstonia solanacearum）导致全世界茄科作物生产严重损失，包括番茄、马铃薯和茄子。为了有效预防疫情爆发，有必要了解病原体和微生物组之间复杂的相互作用。增强微生物组功能的一种有前景的机制是铁载体介导的竞争，这是由根际铁可用性低造成的。本研究探讨了铁竞争在确定微生物组功能中的关键作用及其设计高性能微生物组工程策略的潜力。我们研究了铁载体介导的相互作用对假单胞菌功效的影响。联合体在体外和体内抑制青枯菌。我们的研究结果表明，铁载体的产生显着增强了假单胞菌菌株对病原体生长的抑制作用，而其他代谢物在铁限制条件下效果较差。此外，铁载体在形成菌群内部的相互作用方面发挥着至关重要的作用，最终决定了对青枯病的保护水平。这项研究强调了铁载体在介导联合体相互作用中的关键作用及其对番茄健康的影响。我们的结果还强调了其他次生代谢产物在铁有限环境中的有限功效，强调了铁载体介导的竞争在维持番茄健康和抑制疾病方面的重要性。