Effects of Various Concentrations of Pronase on Flow Cytometric Crossmatching Patients Treated With Rituximab and Donor HLA-Specific Antibodies.,Annals of Laboratory Medicine

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Effects of Various Concentrations of Pronase on Flow Cytometric Crossmatching Patients Treated With Rituximab and Donor HLA-Specific Antibodies.
Annals of Laboratory Medicine ( IF 4.0 ) Pub Date : 2024-07-12 , DOI: 10.3343/alm.2024.0132
Tae-Shin Kim ₁ , Inseong Oh _{1,

2} , Yu Jung Choi ₁ , Minjeong Nam ₃ , Hajeong Lee ₄ , Eun Young Song _{1,

2}

Affiliation

Pronase pretreatment can reduce rituximab (RTX) interference by degrading CD20 in B-cell flow cytometry crossmatch (FCXM) testing. However, it may also reduce the assay sensitivity by degrading HLA molecules. We investigated the effects of various pronase concentrations on RTX interference and the analytical sensitivity of B-cell FCXM testing.

中文翻译：

不同浓度的链霉蛋白酶对利妥昔单抗和供体 HLA 特异性抗体治疗的流式细胞术交叉配血患者的影响。

链霉蛋白酶预处理可以通过降解 B 细胞流式细胞术交叉配型术（FCXM）检测中的 CD20 来减少利妥昔单抗（RTX）干扰。然而，它也可能通过降解 HLA 分子来降低检测灵敏度。我们研究了各种链霉蛋白酶浓度对 RTX 干扰和 B 细胞 FCXM 检测分析敏感性的影响。

更新日期：2024-07-12

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