Vallisneria spinulosa Yan (V.spinulosa Yan) with good ability of water purification is often used for ecological restoration of polluted water bodies. However, it is difficult to survive in turbid water bodies due to the low lighting condition. This study explored the feasibility of introducing artificial underwater light source into water bodies with high turbidity to strengthen the water restoration by V.spinulosa Yan. Addition of underwater light source promoted the clonal reproduction ability of V.spinulosa Yan, thus enhancing the removal loads of total nitrogen (TN), total phosphorus (TP), and nitrate nitrogen (\({\mathrm{NO}}^{-}_{3}{\mbox{-}}{\mathrm{N}}\)) by 1.60–3.43 × 10⁻², 1.49–3.49 × 10⁻³, and 0.80–2.06 × 10⁻²g m⁻² d⁻¹, respectively. Underwater light source significantly reduced the abundance of microbial community on V.spinulosa Yan leaves, as well as most nitrifying bacteria (Nitrosomonadaceae) and denitrifying bacteria (Nitrospira, Comamonadaceae, and Rhodocyclaceae) in the system. But the attachment of some Cyanophyta (Chloroplast and Cyanobacteria) and Photosynthetic bacteria (Rhodobacter) onto the leaves and the growth of Methyloligellaceae in water and sediments were promoted. Nitrogen and phosphorus removal by the growth system of V.spinulosa Yan without underwater light source mainly depended on the biological processes by functional bacteria, while the absorption and co-assimilation effect of V.spinulosa Yan with underwater light source.

苦草(V.spinulosa Yan)具有良好的水质净化能力，常用于污染水体的生态修复。但由于光照条件较差，在浑浊水体中生存困难。本研究探讨了在高浊度水体中引入人工水下光源强化刺蔓藻水体修复的可行性。水下光源的加入促进了V.spinulosa Yan的克隆繁殖能力，从而提高了总氮（TN）、总磷（TP）和硝态氮的去除负荷（\({\mathrm{NO}}^{- }_{3}{\mbox{-}}{\mathrm{N}}\)) 乘以 1.60–3.43 × 10 ⁻² 、 1.49–3.49 × 10 ⁻³ ，以及分别为 0.80–2.06 × 10 ⁻² g m ⁻² d ⁻¹ 。水下光源显着降低了V.spinulosa Yan叶片上微生物群落的丰度，以及系统中大多数硝化细菌（亚硝化单胞菌科）和反硝化细菌（硝化螺菌属、丛毛单胞菌科和红环菌科）的丰度。但一些蓝藻门（叶绿体和蓝细菌）和光合细菌（红细菌）附着在叶子上，促进了水和沉积物中甲基寡糖科的生长。无水下光源条件下刺葡萄生长系统的脱氮除磷主要依靠功能菌的生物过程，而有水下光源条件下刺葡萄生长系统的脱氮除磷主要依靠功能菌的生物过程。