Eye depth is an important agronomic trait affecting tubers' appearance, quality, and processing suitability. Hence, cultivating varieties with uniform shapes and shallow eye depth are important goals for potato breeding. In this study, based on the primary mapping of the tuber eye-depth locus using a small primary-segregating population, a large secondary-segregating population with 2,100 individuals was used to map the eye-depth locus further. A major quantitative trait locus for eye-depth on chromosome 10 was identified (designated ) using BSA-seq and traditional QTL mapping method. The could explain 55.0% of the eye depth phenotypic variation and was further narrowed to a 309.10 kb interval using recombinant analysis. To predict candidate genes, tissue sectioning and RNA-seq of the specific tuber tissues were performed. Genes encoding members of the peroxidase superfamily with likely roles in indole acetic acid regulation were considered the most promising candidates. These results will facilitate marker-assisted selection for the shallow-eye trait in potato breeding and provide a solid basis for eye-depth gene cloning and the analysis of tuber eye-depth regulatory mechanisms.

中文翻译：

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马铃薯块茎眼深度精细定位及候选基因分析


眼深是影响块茎外观、品质和加工适宜性的重要农艺性状。因此，培育形状均匀、眼深浅的品种是马铃薯育种的重要目标。在本研究中，基于使用小型初级分离种群对块茎眼深轨迹进行初步测绘，使用包含 2,100 个体的大型次级分离种群进一步绘制眼深轨迹。使用 BSA-seq 和传统的 QTL 作图方法鉴定了 10 号染色体上眼睛深度的主要数量性状位点（指定为 ）。这可以解释 55.0% 的眼睛深度表型变异，并使用重组分析进一步缩小到 309.10 kb 区间。为了预测候选基因，对特定块茎组织进行了组织切片和 RNA 测序。编码可能在吲哚乙酸调节中发挥作用的过氧化物酶超家族成员的基因被认为是最有希望的候选者。这些结果将有助于马铃薯育种中浅眼性状的标记辅助选择，并为眼深基因克隆和块茎眼深调控机制分析提供坚实的基础。