Turnip mosaic virus (TuMV) constitutes one of the primary diseases affecting , severely impacting its production and resulting in crop failures in various regions worldwide. Recent research has demonstrated the significance of plant translation initiation factors, specifically the and family genes, as essential recessive disease resistance genes. In our study, we conducted evolutionary and gene expression studies, leading us to identify as a potential TuMV-resistant gene. Leveraging CRISPR/Cas9 technology, we obtained mutant plants with edited gene. We confirmed confers resistance against TuMV through phenotypic observations and virus content evaluations. Furthermore, we employed ribosome profiling assays on mutant seedlings to unravel the translation landscape in response to TuMV. Interestingly, we observed a moderate correlation between the fold changes in gene expression at the transcriptional and translational levels ( = 0.729). Comparative analysis of ribosome profiling and RNA-seq data revealed that plant–pathogen interaction, and MAPK signaling pathway–plant pathways were involved in -mediated TuMV resistance. Further analysis revealed that sequence features, coding sequence length, and normalized minimal free energy, influenced the translation efficiency of genes. Our study highlights that the loss of can result in a highly intricate translation mechanism, acting synergistically with transcription to confer resistance against TuMV.

中文翻译：

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编辑 eIF(iso)4E.c 赋予白菜对芜菁花叶病毒的抗性


芜菁花叶病毒（TuMV）是影响芜菁的主要病害之一，严重影响芜菁的生产，导致世界各地农作物歉收。最近的研究证明了植物翻译起始因子，特别是 和 家族基因，作为必需的隐性抗病基因的重要性。在我们的研究中，我们进行了进化和基因表达研究，使我们确定了潜在的 TuMV 抗性基因。利用CRISPR/Cas9技术，我们获得了基因编辑的突变植物。我们通过表型观察和病毒含量评估证实对 TuMV 具有抗性。此外，我们对突变幼苗进行了核糖体分析，以揭示对 TuMV 的翻译情况。有趣的是，我们观察到转录和翻译水平上基因表达倍数变化之间存在中等相关性（= 0.729）。核糖体分析和 RNA-seq 数据的比较分析表明，植物 - 病原体相互作用以及 MAPK 信号通路 - 植物通路参与介导的 TuMV 抗性。进一步分析表明，序列特征、编码序列长度和归一化最小自由能影响基因的翻译效率。我们的研究强调，缺失可能导致高度复杂的翻译机制，与转录协同作用，从而赋予对 TuMV 的抗性。