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Closed Bipolar Nanoelectrode Array for Ultra-Sensitive Detection of Alkaline Phosphatase and Two-Dimensional Imaging of Epidermal Growth Factor Receptors
ACS Sensors ( IF 8.2 ) Pub Date : 2024-07-06 , DOI: 10.1021/acssensors.4c00918 Jiao Gao 1 , Hua-Jiang Jin 1 , Xuan Wei 1 , Xin-Lei Ding 1 , Zhong-Qiu Li 1 , Kang Wang 1 , Xing-Hua Xia 1
ACS Sensors ( IF 8.2 ) Pub Date : 2024-07-06 , DOI: 10.1021/acssensors.4c00918 Jiao Gao 1 , Hua-Jiang Jin 1 , Xuan Wei 1 , Xin-Lei Ding 1 , Zhong-Qiu Li 1 , Kang Wang 1 , Xing-Hua Xia 1
Affiliation
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The combination of closed bipolar electrodes (cBPE) with electrochemiluminescence (ECL) imaging has demonstrated remarkable capabilities in the field of bioanalysis. Here, we established a cBPE-ECL platform for ultrasensitive detection of alkaline phosphatase (ALP) and two-dimensional imaging of epidermal growth factor receptor (EGFR). This cBPE-ECL system consists of a high-density gold nanowire array in anodic aluminum oxide (AAO) membrane as the cBPE coupled with ECL of highly luminescent cadmium selenide quantum dots (CdSe QDs) luminophores to achieve cathodic electro-optical conversion. When an enzyme-catalyzed amplification effect of ALP with 4-aminophenyl phosphate monosodium salt hydrate (p-APP) as the substrate and 4-aminophenol (p-AP) as the electroactive probe is introduced, a significant improvement of sensing sensitivity with a detection limit as low as 0.5 fM for ALP on the cBPE-ECL platform can be obtained. In addition, the cBPE-ECL sensing system can also be used to detect cancer cells with an impressive detection limit of 50 cells/mL by labeling ALP onto the EGFR protein on A431 human epidermal cancer cell membranes. Thus, two-dimensional (2D) imaging of the EGFR proteins on the cell surface can be achieved, demonstrating that the established cBPE-ECL sensing system is of high resolution for spatiotemporal cell imaging.
中文翻译：
用于超灵敏检测碱性磷酸酶和表皮生长因子受体二维成像的闭合双极纳米电极阵列
闭合双极电极（cBPE）与电化学发光（ECL）成像的结合在生物分析领域展现了非凡的能力。在这里，我们建立了cBPE-ECL平台，用于超灵敏检测碱性磷酸酶（ALP）和表皮生长因子受体（EGFR）的二维成像。该cBPE-ECL系统由阳极氧化铝（AAO）膜中的高密度金纳米线阵列组成，作为cBPE，与高发光硒化镉量子点（CdSe QD）发光体的ECL相结合，以实现阴极电光转换。当以4-氨基苯磷酸一钠盐水合物（p-APP）为底物，4-氨基苯酚（p-AP）为电活性探针时，引入ALP的酶催化放大效应，检测灵敏度显着提高在 cBPE-ECL 平台上可以获得低至 0.5 fM 的 ALP 限制。此外，cBPE-ECL传感系统还可以通过将ALP标记到A431人表皮癌细胞膜上的EGFR蛋白上来检测癌细胞，检测限高达50个细胞/mL。因此，可以实现细胞表面EGFR蛋白的二维（2D）成像，证明所建立的cBPE-ECL传感系统对于时空细胞成像具有高分辨率。
更新日期：2024-07-06
中文翻译：
用于超灵敏检测碱性磷酸酶和表皮生长因子受体二维成像的闭合双极纳米电极阵列
闭合双极电极（cBPE）与电化学发光（ECL）成像的结合在生物分析领域展现了非凡的能力。在这里，我们建立了cBPE-ECL平台，用于超灵敏检测碱性磷酸酶（ALP）和表皮生长因子受体（EGFR）的二维成像。该cBPE-ECL系统由阳极氧化铝（AAO）膜中的高密度金纳米线阵列组成，作为cBPE，与高发光硒化镉量子点（CdSe QD）发光体的ECL相结合，以实现阴极电光转换。当以4-氨基苯磷酸一钠盐水合物（p-APP）为底物，4-氨基苯酚（p-AP）为电活性探针时，引入ALP的酶催化放大效应，检测灵敏度显着提高在 cBPE-ECL 平台上可以获得低至 0.5 fM 的 ALP 限制。此外，cBPE-ECL传感系统还可以通过将ALP标记到A431人表皮癌细胞膜上的EGFR蛋白上来检测癌细胞，检测限高达50个细胞/mL。因此，可以实现细胞表面EGFR蛋白的二维（2D）成像，证明所建立的cBPE-ECL传感系统对于时空细胞成像具有高分辨率。
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