Oxygen-tolerant, eosin Y mediated synthesis of protein–polymer biohybrids and protein-coated polymer nanoparticles,Polymer Chemistry

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Oxygen-tolerant, eosin Y mediated synthesis of protein–polymer biohybrids and protein-coated polymer nanoparticles
Polymer Chemistry ( IF 4.1 ) Pub Date : 2024-07-10 , DOI: 10.1039/d4py00407h
Errika Voutyritsa _{1,

2,

3} , Thomai Lazou ₁ , Jonida Bushi ₁ , Stavroula Margaritaki ₁ , Myrto Charitaki ₁ , Sune M. Christensen ₄ , Nikos S. Hatzakis _{2,

3} , Kelly Velonia ₁

Affiliation

To avoid metal catalysts that are commonly used in conventional approaches for the synthesis of protein–polymer conjugates, an eosin Y/TEMED mediated, photoinduced polymerization of vinyl monomers was optimized. This oxygen tolerant, photoinduced approach allowed the grafting of a series of hydrophobic, hydrophilic and responsive polymers with quantitative protein macroinitiator consumption. CALB bioconjugates were also synthesized and found to retain part of the parent protein activity for extended periods of time. Notably, when BSA was used in the absence of an initiator, protein-coated nanoparticles were shown to form during emulsion polymerization.

中文翻译：

耐氧、曙红 Y 介导的蛋白质-聚合物生物杂化物和蛋白质包被的聚合物纳米粒子的合成

为了避免合成蛋白质-聚合物缀合物的传统方法中常用的金属催化剂，对曙红 Y/TEMED 介导的乙烯基单体的光诱导聚合进行了优化。这种耐氧、光诱导的方法允许接枝一系列疏水性、亲水性和响应性聚合物，并消耗定量的蛋白质大分子引发剂。还合成了 CALB 生物结合物，发现其可以长时间保留部分母蛋白活性。值得注意的是，当在没有引发剂的情况下使用 BSA 时，在乳液聚合过程中会形成蛋白质包被的纳米颗粒。

更新日期：2024-07-10

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