Early detection of hypo/hyperglycemia using a microneedle electrode array-based biosensor for glucose ultrasensitive monitoring in interstitial fluid,Lab on a Chip

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Early detection of hypo/hyperglycemia using a microneedle electrode array-based biosensor for glucose ultrasensitive monitoring in interstitial fluid
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-07-10 , DOI: 10.1039/d4lc00365a
Samar H Tawakey _{1,

2} , Mohammad Mansour ₁ , Ahmed Soltan ₁ , Alyaa I Salim ₃

Affiliation

Diabetes is a common chronic metabolic disease with a wide range of clinical symptoms and consequences and one of the main causes of death. For the management of diabetes, painless and continuous interstitial fluid (ISF) glucose monitoring is ideal. Here, we demonstrate continuous diabetes monitoring using an integrated microneedle (MN) biosensor with an emergency alert system. MNs are a novel technique in the field of biomedical engineering because of their ability to analyze bioinformation with minimal invasion. In this work we developed a poly(methyl methacrylate) (PMMA) based MN glucose sensor. The device was produced by the 3D printing technique, microfabrication, electrodeposition, and enzyme immobilization step. The in vitro test for the glucose MN sensor showed a linear range from 1.5 to 14 mM with a sensitivity of 1.51 μA mM⁻¹, limit of detection (LOD) of 0.35 mM and good selectivity. Highly repeatable sensing is observed with good reproducibility. The interference-free detection of glucose in the presence of physiologically relevant concentrations of ascorbic acid, uric acid, and mannose is demonstrated, along with the operational stability of the array. After resolving the biofouling consequences linked to on-body sensing, this MN platform would be appealing for minimally invasive electrochemical glucose monitoring. An alert is sent to confidants via email or SMS when the values are abnormal. The application is also able to display the recorded values in the form of a graph to help determine the state of health of the user over a period of time. It can be concluded that continuous monitoring and an emergency alert system are important for keeping an eye on diabetic patients and can send alert in case of an abnormal situation of the patient.

中文翻译：

使用基于微针电极阵列的生物传感器早期检测低/高血糖，用于间质液中的葡萄糖超灵敏监测

糖尿病是一种常见的慢性代谢性疾病，具有广泛的临床症状和后果，是导致死亡的主要原因之一。对于糖尿病的管理，无痛连续间质液 (ISF) 血糖监测是理想的选择。在这里，我们演示了使用带有紧急警报系统的集成微针 (MN) 生物传感器进行连续糖尿病监测。 MN 是生物医学工程领域的一项新技术，因为它们能够以最小的侵入来分析生物信息。在这项工作中，我们开发了一种基于聚甲基丙烯酸甲酯 (PMMA) 的 MN 葡萄糖传感器。该装置是通过3D打印技术、微加工、电沉积和酶固定步骤生产的。葡萄糖MN传感器的体外测试显示线性范围为1.5至14 mM，灵敏度为1.51 μA mM ^-1 ，检测限(LOD)为0.35 mM，并且选择性良好。观察到高度可重复的传感，具有良好的再现性。证明了在存在生理相关浓度的抗坏血酸、尿酸和甘露糖的情况下对葡萄糖的无干扰检测，以及阵列的操作稳定性。在解决与体感传感相关的生物污染后果后，该 MN 平台将对微创电化学葡萄糖监测具有吸引力。当值异常时，会通过电子邮件或短信向好友发送警报。该应用程序还能够以图表的形式显示记录的值，以帮助确定用户在一段时间内的健康状况。由此可见，持续监测和紧急警报系统对于密切关注糖尿病患者非常重要，并且可以在患者出现异常情况时发出警报。

更新日期：2024-07-10

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