Recent Innovations and Technical Advances in High-Throughput Parallel Single-Cell Whole-Genome Sequencing Methods,Small Methods

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Recent Innovations and Technical Advances in High-Throughput Parallel Single-Cell Whole-Genome Sequencing Methods
Small Methods ( IF 10.7 ) Pub Date : 2024-07-09 , DOI: 10.1002/smtd.202400789
Yi Qiao ₁ , Tianguang Cheng ₁ , Zikun Miao ₁ , Yue Cui ₁ , Jing Tu ₁

Affiliation

Single-cell whole-genome sequencing (scWGS) detects cell heterogeneity at the aspect of genomic variations, which are inheritable and play an important role in life processes such as aging and cancer progression. The recent explosive development of high-throughput single-cell sequencing methods has enabled high-performance heterogeneity detection through a vast number of novel strategies. Despite the limitation on total cost, technical advances in high-throughput single-cell whole-genome sequencing methods are made for higher genome coverage, parallel throughput, and level of integration. This review highlights the technical advancements in high-throughput scWGS in the aspects of strategies design, data efficiency, parallel handling platforms, and their applications on human genome. The experimental innovations, remaining challenges, and perspectives are summarized and discussed.

中文翻译：

高通量并行单细胞全基因组测序方法的最新创新和技术进展

单细胞全基因组测序（scWGS）从基因组变异方面检测细胞异质性，这些变异是可遗传的，在衰老和癌症进展等生命过程中发挥着重要作用。高通量单细胞测序方法最近的爆炸性发展使得通过大量新策略实现高性能异质性检测成为可能。尽管总成本有限，但高通量单细胞全基因组测序方法的技术进步是为了实现更高的基因组覆盖率、并行通量和集成水平。本综述重点介绍了高通量 SCWGS 在策略设计、数据效率、并行处理平台及其在人类基因组上的应用等方面的技术进步。总结和讨论了实验创新、剩余挑战和观点。

更新日期：2024-07-09

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